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The Role of TEF-1 Family Transcription Factors in The Cardiovascular System.

Research Project

Project/Area Number 10670671
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKumamoto University

Principal Investigator

YASUNAMI Michio  Kumamoto University, School of Medicine, Lecturer, 医学部, 講師 (80244127)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsTranscription factor / Cell differentiation / Atherosclerosis / 筋特異的遺伝子発現 / 血管平滑筋細胞
Research Abstract

The TEF-1 transcription factor family proteins share the TEA domain. Transcriptional enhancer factor-1 (TEF-1) was originally identified as a transcriptional activator which binds to both GT-IIC and Sph motifs of SV40 enhancer. Later studies have shown that TEF-1 and/or similar factor (s) bind to M-CAT element in the regulatory region of several muscle-specific genes, and that a mutation of TEF-1 results in severe impairment in cardiac development and mid-gestational lethality presumably due to congestive heart failure. We identified three novel members of TEF-1 family in mouse : Embryonic TEA domain-containing factor (ETF), ETFR-1 and ETFR-2. mRNAs of ETFR-1 and ETFR-2 were expressed in many adult tissues including the heart and vascular smooth muscle cells. In C2C12 myoblast differentiation, the elevated expression of ETFR-2 mRNA associated with the myotube formation and sarcomeric myosin expression. ETFR-2 mRNA was also induced by growth factors in serum-depleted culture of primary … More rat aortic smooth muscle cells. Thus ETFR-2 as well as TEF-1 may contribute to the regulation of myocyte development.
Although a variety of genes are known to have one or more binding sites for the TEF-1 family proteins in their regulatory regions, the molecular mechanism of transcriptional activation is largely unknown. Recently, it was reported that the Drosophila TEF-1 homologue, Scalloped protein (Sd) mediates the activation of wing imaginal disc-specific gene transcription by means of the direct interaction with the wing disc-specific Vestigial protein (Vg). Further, The existence of nucleotide sequences coding for human and mouse proteins which show the amino acid sequence similarity to the Sd-binding domain of Vg was also reported. We isolated cDNA fragments coding for mouse Vg-related factors (VRF) by RT-PCR.The expression of the mouse VRF mRNA was detected in the placenta, and to lesser extent in the embryo and testis. The general mechanism of transcriptional regulation by TEF-1 family still remains to be elucidated. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Oda,H. et al.: "Structure of the mouse NDRF gene and its regulation during neuronal differentiation of P19 cells."Molecular Brain Res.. 77. 37-46 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Oda, H.et al.: "Structure of the mouse NDRF gene and its regulation during neuronal differentiation of P19 cells."Vol.77. 37-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto,K.et al.: "Cloning from insulinoma cells of synapsin I associated with insulin secretory granules."J.Biol.Chem.. 274. 2053-2059 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iwata,I.et al.: "Association of polymorphism in the NeuroD/BETA2 gene with insulin dependent diabetes mellitus in Japanese."Diabetes. 48. 416-419 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Oda,H.et al.: "Structure of the mouse NDRF gene and its regulation during neuronal differentiation of P19 cells."Molecular Brain Research. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto,K. et. al.: "Cloning from insulinoma cells of synapsin I asociated with insulin secretory granules." J.Biol.Chem.274. 2053-2059 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwata,I. et al.: "Association of polymorphism in the NeuroD/BETA2 gene with insulin dependent diabetes mellitus in Japanese." Diabetes. 48. 416-419 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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