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Analysis on local blood flow, metabolic NADH distribution and micro-mechanical dysfunction during ischaemia of coronary micro-circulation

Research Project

Project/Area Number 10670686
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKawasaki Medical School

Principal Investigator

OGASAWARA Yasuo  Medical Engineering, Kawasaki Medical School, Associate Professor, 医学部, 助教授 (10152365)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Takeshi  Medical Engineering, Kawasaki College of Allied Health Professions, Associate Professor, 臨床工学科, 助教授 (30249560)
YADA Toyotaka  Medical Engineering, Kawasaki Medical School, Assistant Professor, 医学部, 講師 (00210279)
KAJIYA Fumihiko  Medical Engineering, Kawasaki Medical School, Professor, 医学部, 教授 (70029114)
YAMAMORI Shinji  R&D Center, Nihon Kohden Co., Manager, R&Dセンター, 課長
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsNADH / coronary microcirculation / local blood flow / metabolism imaging / micro-strain / molecular tracer / ischaemia
Research Abstract

We analyzed ischaemia of myocardial micro-circulation and spatial heterogeneity of mitochondrial dysfunction by direct evaluation of NADH fluorescent image in coronary micro-circulation and measurement of capillary blood flow distribution using molecular blood flow tracer. NADH fluorescence imaging system has a CCD camera with 400 thousand pixels to observe the emission fluorescence (420 - 570 nm) against ex-citation ultraviolet ray. The advantages of this system are easy observation by fluorescence for NADH dynamics and high spatial resolution with high time resolution. Blood flow distribution imaging was achieved with molecular blood flow tracer (3H-Desmethylimipramine) with 100 mm spatial resolution. Experiment was performed by 60 seconds hypoxic perfusion in isolated rat heart perfusion preparation. During the experiment, NADH fluorescence from the heart was recorded and molecular blood flow tracers were administered. The findings of the present study were; NADH intensity increased in proportion to the intra-mitochondrial NADH amount immediately after hypoxic perfusion and patchy patterns appeared with mixture of high and low intensity of NADH (several hundreds microns in size). Simultaneous measurement of blood flow distribution by molecular tracers also showed spatial heterogeneity and the size of each spotty area was in order of several hundred microns, which was almost identical with the patch area observed by NADH fluorescence. Furthermore, micro-strain measured at the level of myocardial micro-circulation with the size of a hundred microns also showed heterogeneity on the surface of a heart. Taken together, there is a relationship in distribution among blood flow, metabolic dysfunction and micro-strain. This indicates that hetrogenous myocardial ischaemic area is closely related with blood distribution and mitochondrial metabolic dysfunction.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yasuo Ogasawara: "Imaging of medical metabolic activity by mitochondorial NADH fluorescence in a beating rat heart"Proceedings of 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. 20. 2881-2883 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 梶田達也: "心筋局所代謝評価価のためのNADH蛍光イメージングシステムの試作"電子情報通信学会技術研究報告. 98. 127-132 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小笠原康夫: "心筋微小循環領域における代謝機能と血流分布のイメージング"第14回生体・生理工学シンポジウム論文集. 209-212 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 梶田達也: "NADH蛍光イメ-ジングと分子トレーサイメージングを用いた局所心筋代謝と血流分布の空間的比較検討"電子情報通信学会技術研究報告. 99. 107-114 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小笠原康夫: "虚血心筋における局所代謝イメージング-代謝異常の時・空間的不均一性の可視化"脈管学. 39. 813-817 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小笠原康夫: "NADH蛍光イメ-ジング法と分子トレーサー法による局所心筋代謝と血流分布の空間的比較"第47回レオロジー討論会講演要旨集. 9-10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasuo Ogasawara et al.: "Imaging of medical metabolic activity by mitochondorial NADH fluorescence in a beating rat heart"Proceedings of 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. 20. 2881-2883 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fumihiko Kajiya: "Functions of coronary microcirculation"Microcirculation Annual 1998. 21-22 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasuo Ogasawara et al.: "Evaluation of NADH fluorescence in a beating heart by fluorescence CCD system"Third World Congress of Biomechanics Abstracts. 205 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Eiji Toyota et al.: "Myocardial microstrains and flow dynamics of in vivo canine heart visualized by newly developed high-speed CCD intravital videomicroscope"Third World Congress of Biomechanics Abstracts. 66 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeshi Matsumoto et al.: "Vascular segmental regulation in the coronary microcirculation under inhibition of nitric oxide synthesis"Third World Congress of Biomechanics Abstracts. 64 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Eiji Toyota et al.: "Myocardial micro-deformation greatly affects coronary inflow into capillary network in in vivo canine heart"Circulation. 98(Suppl.). I-489 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeshi Matsumoto et al.: "Transmural microcirculatory blood flow distribution in the right and left ventricular free walls of rabbits"American Journal of Physiology. 277. H183-191 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Eiji Toyota et al.: "Hterogeneity of myocardial micro-deformation at the size minimum control unit of coronary flow in beating canine heart"Circulation. 100(Suppl.). I-556 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小笠原 康夫: "心筋微小循環領域における代謝機能と血流分布のイメージング"第14回生体・生理工学シンポジウム論文集. 209-212 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 梶田達也: "NADH蛍光イメージングと分子トレーサイメージングを用いた局所心筋代謝と血流分布の空間的比較検討"電子情報通信学会技術研究報告. 99. 107-114 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 小笠原 康夫: "虚血心筋における局所代謝イメージング-代謝異常の時・空間的不均一性の可視化-"脈管学. 39. 813-817 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 梶田達也: "ミトコンドリアNADH蛍光法による心筋の局所代謝機能評価-正常と糖尿病ラットでの比較-"医用電子と生体工学. 37(suppl). 492 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 小笠原 康夫: "NADH蛍光イメージング法と分子トレーサー法による局所心筋代謝と血流分布の空間的比較"第47回レオロジー討論会講演要旨集. 9-10 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takeshi Matsumoto: "Transmural microcirculatory blood flow distribution in the right and left ventricular free walls of rabbits"American Journal of physiology. 277. H183-H191 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 梶田達也: "心筋局所代謝評価のためのNADH蛍光イメージングシステムの試作" 電子情報通信学会技術研究報告. 98-174. 127-132 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasuo Ogasawara: "Imaging of medical metabolic activity by mitochordorial NADH fluoregence in a beating rat heart" Proceedings of 20th Annual Interhational Conference of the IEEE Engineering in Medicine and Brology Society. 20. 2881-2883 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小笠原 康夫: "NADH蛍光法による拍動心筋虚血イメージング" 医用電子と生体工学. 36・suppl.452 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasuo Ogasawara: "Evaluation of NADH fluorescence in a beating heart by fluorescence CCD system" Third World Congress of Biomechanics Abstracts. 205 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小笠原 康夫: "虚血心筋における局所代謝イメージング-代謝異常の時・空間的不均一性の可視化-" 脈管学. 38-8. 531 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小笠原 康夫: "NADH蛍光イメージングシステムによる心筋局所代謝機能の評価" 第21会日本バイオレオロジー学会年会抄録. 63 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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