| Project/Area Number |
10670726
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Osaka City University Medical School (1999)
Shiga University of Medical Science (1998) |
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Principal Investigator |
YAMANO Tsunekazu Osaka City University Medical School, Department of Pediatrics, Professor, 医学部, 教授 (20093172)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | MALFORMED BRAIN / HETEROTOPIA / HIPPOCAMPUS / NEURONAL NET-WORK / CATECHOLAMINERGIC FIBER / SEROTONERGIC NERVE FIBER / シナプス |
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| Research Abstract |
This study was undertaken to elucidate neuronal net-work in malformed brain using animal model. Pregnant mice were injected intraperitoneally with cytosine arabinoside on days 13 and 14 of pregnancy. Brains of their offspring were studied morphologically. They demonstrated heterotopic gray matter (HGM) in the cerebral cortex and heterotopic neuronal cluster in the molecular layer of hippocampus CA1, in addition to microcephaly. Arrangement of neurons and directions of their stem dendrites in HGM were very irregular by Golgi-Cox staining, but synapses on them were formed until day 20 after birth by electronmicroscopy. Immunohistochemical examination, using anti-tyrosine hydroxylase or anti-serotonin antibodies, showed that TH immunoreactive fibers did not innervate into HGM, but serotonin immunoreactive fibers were distributed in HGM. Distribution of both fibers in HGM was same to that in the pyramidal cell layer in hippocampus. Examination serial sections of HGM by HE staining in mice on day 2 demonstrated that neurons, which should migrate to the pyramidal cell layer in hippocampus, were arrested in the cerebral cortex, forming HGM. These results indicated that catecholaminergic fibers and serotonergic fibers extend for essential postsynaptic elements in even malformed brain.
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