| Project/Area Number |
10670740
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
FUSE Shigeto (1999) Sapporo Medical University, Dept. of Pediatrics, Instructor, 医学部・小児科, 助手 (30274921)
富田 英 (1998) 札幌医科大学, 医学部, 講師 (60180171)
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| Co-Investigator(Kenkyū-buntansha) |
HATAKEYAMA Kinya Sapporo Medical University, Dept. of Pediatrics, Instructor, 医学部・小児科, 研究生
布施 茂登 札幌医科大学, 医学部, 助手 (30274921)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | congestive liver / Protein C / Rat / mRNA / Fontan procedure / Blood coagulation / 肝うっ血 / 右心不全 / 血液凝固因子 / プロテインC / 肝静脈圧 / RT-PCR法 / ネステッドPCR法 |
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| Research Abstract |
Purpose: We developed novel liver congestion model of rat. We studied the relationship between infra vena caval pressure and expression of Protein C mRNA, which is most important blood coagulation inhibitor.
Method: Subjects were 6-8 weeks age male Wister rattus. We set control (C) group was sham-operated, and High infra vena caval pressure (H) group was made by continuous dextran infusion. We made Protein C primer and measured protein C RNA value by beta-action.
Results: IVC pressure of group C was 1 cmH20, and the group H was 3cmH20. Protein C value of the group C were 1.40 and 1.19, and group H were 0.58 and 1.05.
Discussion: Protein C value was lower in the group H than the group C. We demonstrated low protein C production in high IVC pressure using this new rat model. This suggests that low serum protein C after Fontan procedure results from high central venous pressure or high hepatic venous pressure. We could estimate the low protein C level in high IVC pressure patients, that may lead to coagulopathy.
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