| Project/Area Number |
10670829
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUZUKI Norio University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (10010050)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Keiichi University of Tokyo, Graduate School of Medicine, Lecturer, 医学部・附属病院, 講師 (80188896)
HIRANO Kazuya University of Tokyo, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (80251221)
HOSOI Yoshio University of Tokyo, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (50238747)
SAKAI Kazuo Central Research Institute of Electric Power Industry, Senior Research Scientist, 生物科学部, 室長(研究職) (40153837)
ITOH Masamitsu Yokohama Posts and Telecommunications Hospital, Medical & Research Associate, 医師(研究職) (80176362)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Radiation pneumonitis / Radiation fibrosis / macrophage |
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| Research Abstract |
Because side effects of lung irradiation, such as lung fibrosis and radiation pneumonitis, occur 6-12 months after irradiation, long experiment period is required for investigation on such side effects using mice or rats.
We investigated effects of Argatroban on lung fibrosis after irradiation. Right lungs of rats were exposed to 17 Gy electron radiation with or without treatment with Argatroban. In the group of rats treated with Argatroban, lung fibrosis was not severe and the numbers of phagocytes invading into the lungs were significantly less compared with those in the non-treated group of rats.
Radiation pneumonitis is usually observed in the irradiated area. However, it has been reported that it sometimes occurs outside the irradiated area. This suggests that not only radiation-induced damage but also the reaction of the irradiated host may cause radiation pneumonitis. Furthermore, radiation pneumonitis was not observed in the intercellular adhesion molecule 1 (ICAM-1) knockout mice, which suggests the involvement of immune response of the irradiated host to radiation pneumonitis. To investigate the role of macrophages in the development of pneumonitis, we investigated the response of macrophages to ionizing radiation in vitro. Significant increases of IL-1 β and IL-6 mRNA were observed 1-2 hours after irradiation, and significant decreases of these mRNA were observed 4 hours after irradiation. We will investigate (1) the expression of TNF- α in macrophages, which is reported to be important for tissue damage, and (2) the protective effects of HGF and Argatroban against lung damage.
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