Fundamental Study on Assessment of Neurotransplantation and Drug Treatment in Alzheimers Disease Using Nuclear Medicine Imaging
| Project/Area Number |
10670832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | KANAZAWA UNIVERSITY |
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Principal Investigator |
MORI Hirofumi Kanazawa University, Radioisotope Center, Professor, アイソトープ総合センター, 教授 (90019604)
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| Co-Investigator(Kenkyū-buntansha) |
TSJI Siro Kanazawa University, Scool of Medicine, Associate Professor, 医学部, 助教授 (70227388)
SHIBA Kazuhiro Kanazawa University, Radioisotope Center, Associate Professor, アイソトープ総合センター, 助教授 (40143929)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Alzheimer's disease / Lesion of Nucleus Basalis Magnocellularis / Acetylcholine / Receptor / Neurotransplantation / Transporter / β-amyloid |
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| Research Abstract |
(1) In this study, β-amyloid protein was infused into the cerebral ventricle of rats for 14 days ; the eight-arm radial maze was used to evaluate memorial ability. In the same animals, vesicular acetylcholine transporter and muscarinic acetylcholine receptor density was measured. The performance of the eight-arm radial maze task was impaired in β-amyloid protein treated rats. In neocortex, the vesicular acetylcholine transporter density was lower in β-amyloid protein-treated rats than vehicle treated rats ; there was no difference in muscarinic acetylcholine receptor density between the two groups. These results suggest that the reduction in vesicular acetylcholine transporter density is related to memory impairment induced by β-amyloid protein.
(2) It is considered that the nodosal ganglion grafting improves the learning and memory disorder in the nucleus basalis magnocellularis lesioned rat, and that [^3H]-vesamicol is one of the mapping agents for presynaptic cholinergic neurons. Quantitative autoradiography was performed using high sensitive imaging plate system to evaluate the effect of neurotransplantation on [^3H]-vesamicol binding. The affected-side to unaffected-side ratio of [^3H]-vesamicol in the nucleus basalis magnocellularis lesioned group was significantly lower than that in the sham-operated group at I , 2 and 4 weeks (p< 0.01). In the transplanted group, the ratio was significantly higher than that in the untreated group at 4 weeks (p<0.05). [^3H]-vesamicol binding is one of the most sensitive indicators of nucleus basalis magnocellularis lesions and the effect of the nodosal ganglion grafting.
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Report
(4 results)
Research Products
(7 results)
