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Mutagenic effects at HPRT locus in Chinese Hamster Ovary(CHO)cells induced by epi-thermal and thermal neutrons

Research Project

Project/Area Number 10670845
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KINASHI Yuko  Kyoto University Research Reactor Institute, Radiation Oncology Research Lab., Instructor, 原子炉実験所, 助手 (80252534)

Co-Investigator(Kenkyū-buntansha) SAKURAI Yoshinori  Kyoto University Research Reactor Institute, Radiation Life Science, Instructor, 原子炉実験所, 助手 (20273534)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsEpithermal neutron / Thermal neutron / Boron neutron capture therapy(BNCT) / PCR / HPRT mutation / CHO cells
Research Abstract

CHO cells were exposed to thermal and epithermal neutrons and the occurrence of mutations at the HPRT locus was measured. To determine mutant frequency and cell survival, cells were irradiated with thermal and epithermal neutrons under three conditions : thermal neutron mode, mixed mode with thermal and epithermal neutrons, and epithermal neutron mode. The mutagenicity was different among the three irradiation modes, with the epithermal neutron mode showing a mutation frequency about 6.3-fold that of the thermal neutron mode and about 1.7-fold that of the mixed mode. In the thermal neutron and mixed mode, boron did not increased significantly the frequency of the mutants at the same dose. Therefore, the effect of boron as in boron neutron capture therapy (BNCT) is quantitatively minimal in terms of mutation induction. Over 300 independent neutron-induced mutant clones were isolated from separate experiments. The molecular structure of HPRT mutations was determined by analysis of all nine exons by multiplex polymerase chain reaction. In the thermal neutron and mixed modes, total and partial deletions were dominant and the fraction of total deletions were increased in the presence of boron. In the epithermal neutron mode, more than half of the mutations observed were total deletions. Our results suggest that there are clear differences between thermal and epithermal neutron beams in their mutagenicity and in the structural pattern of the mutants that they induce.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yuko Kinashi, Yoshinori Sakurai, et al.: "Dimethyl sulfoxide protects against thermal and epithermal neutron induced cell death and mutagenesis of Chinese hamster ovary (CHO) cells"Int. J. Radiation Oncology Biol. Phys. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Yoshinori Sakurai, et al.: "Mutagenic effects at HPRT locus in Chinese hamster ovary (CHO) cells induced by epi-thermal and thermal neutrons"Radiation Research. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Yoshinori Sakurai, et al.: "Mutagenic effects at HPRT locus in Chinese hamster ovary cells induced by epi-thermal and thermal neutrons"Frontiers in Neutron Capture Therapy M. F. Hawthome, K. Shelly, and R. J. Wiersema, eds.. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Mitsuhiro Akaboshi, et al.: "Resistance to 195m Pt-radiolabeled cis-diaminedichloroplatinum(II) of SHOK cells transfected with various oncogenes."Radiation Medicine. 233-237 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Shinichiro Masunaga, et al.: "Hyperthermia enhances Thermal-neutron-induced cell death of human glioblastoma cell lines at low concentration of 10B."Int. J. Radiation Oncology Biol. Phys. 1185-1192 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Yoshinori Sakurai, et al.: "Dimethyl sulfoxide protects against thermal and epithermal neutron induced cell death and mutagenesis of Chinese hamster ovary (CHO) cells."Int. J. Radiation Oncology Biol. Phys.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Yoshinori Sakurai, et al.: "Mutagenic effects at HPRT locus in Chinese hamster ovary (CHO) cells induced by epi-thermal and thermal neutrons."Radiation Research. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Yoshinori Sakurai, et al.: "Mutagenic effects at HPRT locus in Chinese hamster ovary cells induced by epi-thermal and thermal neutrons."Frontiers in Neutron Capture Therapy, M. F. Hawthorne, K. Shelly, and R. J. Wiersema, eds.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Mitsuhiko Akaboshi, et al.: "Resistance to ィイD1195mィエD1Pt-radiolabeled cisdiaminedichloroplatinum (II) of SHOK cells transfected with various oncogenes."Radiation Medicine. 16. 233-237 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi, Shinichiro Masunaga, et al.: "Hyperthermia enhances Thermal-neutroninduced cell death of human glioblastoma cell lines at low concentration of 10B."Int. J. Radiation Oncology Biol. Phys.. 40. 1185-1192 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuko Kinashi,Yoshinori Sakurai et al.: "Dimethyl sulfoxide protects against thermal and epithermal neutron induced cell death and mutagenesis of Chinese hamster ovary (CHO) cells"Int.J.Radiation Oncology Biol.Phys.. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuko Kinashi,Yoshinori Sakurai et al.: "Mutagenic effects at HPRT locus in Chinese hamster ovary (CHO) cells induced by epi-thermal and thermal neutrons"Radiation Research. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuko Kinashi,Yoshinori Sakurai et al.: "Mutagenic effects at HPRT locus in Chinese hamster ovary cells induced by epi-thermal and thermal neutrons"Frontiers in Neutron Capture Therapy M.F.Hawthorne,K.Shelly, and R.J.Wiersema, eds.. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuko Kinashi, Mitsuhiko Akaboshi et al.: "Resistance to ^<195m>Pt-radiolabeled cis-diaminedichloroplatinum(II) of SHOK cells transfected with various oncogenes"Radiation Medicine. 16・4. 233-237 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuko Kinashi,Shinichiro Masunaga et al.: "Hyperthermia enhances Thermal-neutron-induced cell death of human glioblastoma cell lines at low concentration of ^<10>B"Int.J.Radiation Oncology Biol.Phys.. 40・5. 1185-1192 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuko Kinashi,Shinichiro Masunaga,et al.: "Hyperthermia enhances Thermal-neutron-induced cell death of human glioblastoma cell lines at low concentration of ^<10>B." Int.J.Radiation Oncology Biol.Phys.40・5. 1185-1192 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yuko Kinashi,Mitsuhiko Akaboshi,et al.: "Resistance to ^<195m>Pt-radiolabeled cis-diaminedichloroplatinum(II)of SHOK cells transfected with various oncogenes." Radiation Medicine. 16・4. 233-237 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yuko Kinashi,Yoshinori Sakurai,et al.: "Mutagenic effects at HPRT locus in Chinese hamster ovary cells induced by epi-thermal and thermal neutrons" Frontiers in Neutron Capture Therapy M.F.Hawthorne,K.Shelly,and R.J.Wiersema,eds.(1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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