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Establishment of a new non-invasive diagnositic method for the breast cancer by measuring in vivo estrogen receptor concentration.

Research Project

Project/Area Number 10670857
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SASAKI Masayuki  Kyushu University Hospital, Department of Radiology, Assistant, 医学部・附属病院, 助手 (40240907)

Co-Investigator(Kenkyū-buntansha) KOGA Hirofumi  Kyushu Univ Hosp, Radilogy, Medical staff, 医学部・附属病院, 医員
HAYASHI Kazutaka  Kyushu Univ Hosp, Radilogy, Assistant, 医学部・附属病院, 助手 (00325458)
KUWABARA Yasuo  Kyushu Univ Hosp, Radilogy, Associate Professor, 医学部・附属病院, 助教授 (30150436)
NAKAGAWA Makoto  Kyushu Univ Hosp, Radilogy, Medical staff, 医学部・附属病院, 医員
藤原 雅人  九州大学, 医学部, 助手 (30304794)
吉田 毅  九州大学, 医学部, 助手 (40284509)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords^<18>F-estradiol / estrogen receptor / positron emission tomographt (PET) / breast cancer / diagnosis / therapeutic effect
Research Abstract

(1) The aim of this study is to establish a new diagnostic method to measure the in vivo estrogen receptor (ER) concentration non-invasively.
(2) We synthesize 16α-[^<18>F]-fluoro-17β-estradiol (FES) by reacting 16-epiestriol with ^<18>F.Radiochemical purity of FES was 98.2±0.95% and its specific activity was 36.5±1.25GBq/μ mol. The FES solution for intravenous injection was prepared by dissolving the dried FES in 3.2% Tween, 5% ethanol or 5% human serum albumin in saline solution.
(3) We examined the tissue distribution and kinetics of FES in immature female Sprague-Dawley rats. The FES uptake by uterus, an ER-rich tissue, was highly selective and it was 3.29-5.40 %ID/9 at maximum within 60 minutes after administration. Coadministration of unlabeled β-estradiol showed marked depression of uterine FES uptake. The FES uptake by muscle, ER-negative tissue, was less than 0.88 %ID/g.
(4) The whole body radiation dose were 5.97x10^<-6>〜6.08x10^<-6> Gy/MBq.
(5) No side effect was observed durjng 30 days after administration.
(6) We also examined the FES uptake in rat breast tumors induced by 7,12-dimethylbenz (a) anthracene (DMBA) in SD rats. The FES uptake by rat breast tumor was 0.14±0.06 %ID/g at 60 minutes after administration. The FES uptake by rat breast tumor s correlated with the ER concentration measured by in vitro receptor assay (r=0.45, P<0.05).
(7) These results indicate that the FES uptake by tissue is mainly ER mediated and FES is thus considered to be useful for the non-invasive measurement of ER concentration in vivo by positron emission tomography.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Masayuki Sasaki, et al.: "Biodistribution and breast tumoir uptake of 16α-[^<18>F]-fluoro-17β estradiol in rat"Annals of Nuclear Medicine. 14巻・2号. 127-130 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Sasaki, T.Fukumura, Y.Kuwabara, T.Yoshida, M.Nakagawa, Y.Ichiya, K.Masuda: "Biodistribution and breast tumor uptake of 16α[^<18>F]-flroro-17β-estradiol in rat."Annals of Nuclear Medicine. Vol.14, No.2. 127-130 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masayuki Sasaki, et al.: "Biodistribution and breast tumoir uptake of 16α-[^<18>F]-fluoro-17β estradiol in rat"Annals of Nuclear Medicine. 14巻・2号. 127-130 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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