| Project/Area Number |
10670881
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
NAKANO Takashi Research Center of Charged Particle Therapy, Division of Radiation Medicine, National Institute of Radiological Sciences, Researcher, 重粒子治療センター治療・診断部, 研究員 (20211427)
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| Co-Investigator(Kenkyū-buntansha) |
HASEGAWA Masatoshi Gunma University Faculty of Medicine, Dept of Radiology & Radiation Oncology, Lecturer, 医学部・放射線医学教室, 講師 (50251111)
ABE Atuko Research Center of Charged Particle Therapy, Division of Radiation Medicine, National Institute of Radiological Sciences, Researcher, 重粒子治療センター治療・診断部, 研究員 (20280737)
TSUJII Hirohiko Research Center of Charged Particle Therapy, Division of Radiation Medicine, National Institute of Radiological Sciences, Researcher, 重粒子治療センター治療・診断部, 研究員 (50088853)
OKA Kuniyuki Mito Saisei General Hospital, Section of Pathology, Head, 病理部, 部長 (80094241)
TAKAHASHI Masatoshi Saitama Medical Center, Lecturer, 総合医療センター, 講師 (70241883)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Apoptosis / SOD / Radiation / Cervical Cancer / Immunohistochemical |
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| Research Abstract |
1 Correlation between Bax, bcl-2 protein expression and radiation-induced apoptosis
Bax bcl-2 protein expression correlate with radiation-induced apoptosis on 21 cases with the cervical cancer, including 11 cases of stage 2, 8 cases of stage 3, and 2 cases of stage 4 was investigated. Apoptosis was detected by using Apoptag (Oncor), and Apoptotic Index (AI) was calculated. Bax and bcl-2 protein expressions were detected with both anti-Bax antibody (Oncor) and anti-bcl-2 antibody (DAKO)immunohistochemically.
RESULT : Apoptosis was 0.02% before irradiation, and it significantly increased up to 1.2% at 9Gy. The Dositive rate of Bax was 15% before irradiation, and it increased up to 60% at 9Gy. While that of bcl-2 was 15% before irradiation, and it increased to 25% at 9Gy which was no significant increase. Additionally, a significant correlation was observed between Bax expression and apoptosis of the tumor at 9Gy.
CONCLUSION : Bax protein expression significantly correlates with radiation-induced apoptosis of cervical cancer.
2 Correlation between p53, p21 protein expression and radiation-induced apoptosis
Correlation between p53, p21 tumor expression and radiation-induced apoptosis were investigated. Before irradiation, the mean AI of p53 and p21 were 6.60% and 9.24% respectively. Then after 9Gy the mean AI of p53 and p21 significantly increased to 13.9% and 18.0% respectively. A significant positive correlation appeared between p53 protein index and p21 protein index before irradiation. Mean AI, p53 index, and p21 index were 0.98%, 7.33% and 8.77% respectively. As for radiation-induced AI, a significant negative correlation was observed between both AI and p53 or p21. Moreover, a significant positive correlation was observed between both p53 and p21.
CONCLOSION : The cascade of p53 and p21 act as apoptosis inhibitory action in cervical cancer.
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