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The relation between interleukin-6 and adrenergic and opioid receptor mRNA expressions in the brain ; the role of stress response

Research Project

Project/Area Number 10670895
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionMie University

Principal Investigator

KOMORI Teruhisa  Mie University, Hospital, Lecturer, 医学部・附属病院, 講師 (40178380)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsinterleukin-6 / interleukin-6 receptor / α1 adrenocepter / opioid receptor / brain / mRNA / PCR / stress / 脳内サイトカイン / アドレナリン受容体 / RT-PCR / ラット / 脳内インターロイキン-6 / 脳内アドレナリン受容体 / 脳内オピオイド受容体 / 脳内インターロイキン-6受容体
Research Abstract

The reverse transcriptase-polymerase chain reaction was used. After rats had been restrained for 10-240 min, 30- and 60-min stress resulted in a significant fall in the level of α1 adrenoceptor mRNA in the hypothalamus, while significant elevation was noted after 60-, 120- and 240-min stress in the midbrain. When rats had been restrained for 4 h or 4 h per day for 2 or 3 consecutive days, 3-day stress resulted in a significant decrease in the level of α1 adrenoceptor mRNA, whereas single stress did not significantly affect it in the hypothalamus. In the midbrain, the level significantly increased after a single stress, but significantly decreased after repeated stress. The regional differences may be due to involvement of hypothalamic-pituitary-adrenal axis. The exact mechanism of the observed fall in the both regions after repeated stress is not clear and no previous studies have demonstrated such findings. On the expression of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) mRNAs, single stress did not significantly affect the expression of IL-6 mRNA but significantly reduced the level of IL-6R mRNA, while 2- and 3- day stress significantly reduced the both levels in the hypothalamus. In the midbrain, single stress resulted in a significant rise in the level of IL-6 mRNA, and a significant fall in the level of IL-6R mRNA. Two day stress did not significantly affect the both levels but significantly reduced the both levels in the same region. IL-6 might be suppressed by hypothalamic-pituitary-adrenal axis. We cannot as yet explain the mechanism for observed findings on the repeated stress. We are investigating the effects of stress on the opioid receptor subtypes and the effects of agonists and antagonists of opioid and adrenergic receptor subtypes on IL-6 and IL-6R mRNA.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Satoru Miyahara, et al.: "Effects of restraint stress on α1 adrenoceptor mRNA expression in the hypothalamus and midbrain of the rat"Brain Research. 843. 130-135 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoru Miyahara, et al.: "Effects of single and repeated stresses on the expression of mRNA for α1-adrenoceptors in the rat hypothalamus and midbrain"European Journal of Pharmacology. 379. 111-114 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoru Miyahara, et al.: "Effects of repeated stress on expression of interleukin-6(IL-6) and IL-6 receptor mRNA in rat hypothalamus and midbrain"Life Sciences. 66. PL93-98 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoru Miyahara, et al.: "Effects of restraint stress α1 adrenoceptor mRNA expression in the hypothalamus and midbrain of the rat"Brain Research. 843(1-2). 130-135 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoru Miyahara, et al.: "Effects of single and repeated stresses on the expression of mRNA for α1-adrenoceptors in the rat hypothalamus and midbrain"European Journal of Pharmacology. 379(1). 111-114 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoru Miyahara, et al.: "Effects of repeated stress on expression of interleukin-6 (IL-6) and IL-6 receptor mRNAs in rat hypothalamus and midbrain"Life Sciences. 66(6). PL93-PL98 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoru Miyahara,et al.: "Effects of single and repeated stresses on the expression of mRNA for α_1-adrenoceptors in the rat hypothalamus and midbrain"European Journal of Pharmacology. 379・1. 111-114 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Satoru Miyahara,et al.: "Effects of restraint stress on α_1 adrenoceptor mRNA expression in the hypothalamus and midbrain of the rat"Brain Research. 843・1-2. 130-135 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Satoru Miyahara,et al.: "Effects of repeated stress on expression of interleukin(IL-6) and IL-6 receptor mRNAs in rat hypothalamus and midbrain"Life Science. 66・6. PL93-PL98 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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