• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

IMMUNOTHERAPY USING HEAT SHOCK PROTEIN PREPARATIONS OF LEUKEMIC CELLS AFTER SYNGENEIC BONE MARROW TRANSPLANTAION IN MICE.

Research Project

Project/Area Number 10670931
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

TORIMOTO Yoshihiro  Asahikawa Medical College, Third Department of Internal Medicine, Instructor, 医学部, 助手 (00281882)

Co-Investigator(Kenkyū-buntansha) KOHGO Yutaka  ASAHIKAWA MEDICAL COLLEGE, THIRD DEPTARTMENT OF INTERNAL MEDICINE, PROFESSOR, 医学部, 教授 (10133183)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsHert shock protein / HSP / Hematopoietic stem cell transplantation / Tumor Immunology / CD62L / minimal residual dedease / mouse / Immunotherapy
Research Abstract

Heat shock proteins (HSPs) are the molecular chaper one, which bind endogeneous antigenic peptides and bring them to the major histocompatibility complex. Since HSPs chaper on a broad repertoire of endogeneous peptides, and the identification of each tumor specific antigen is not necessary, the strategy using HSPs is more advantageous than other immunological treatment procedures. In this study, we analyzed the immunotherapy with HSP preparations, HSP70 and GP96 derived from syngeneic leukemic cell lines A20, against minimal residual A20 cells during the reconstitution periods of immune system after syngeneic bone marrow transplantation in mice. All mice without immunization died within 60 days after A20 inoculation in this system. Whereas survival days in HSPs immunized mice were significantly prolonged. In addition, depletion of either CD4+ or CD8+ T lymphocyte significantly abrogated this efficacy of HSPs immunization, indicating that immunization with HSPs required CD4+ and CD8+ T lymphocytes in the effector phase of tumor immunity. Moreover, vaccination of HSP preparations derived from A20 elicited responses of potent cytotoxic T lymphocytes activities specific against A20 and led to memory CD4 T cell responses. These observations may suggest that the complex of HSPs and peptide derived from leukemic cells lead to specific immunity for mice with preexisting minimal amount of leukemic cells after syngeneic bone marrow transplantation.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Noriyasu Taya et al: "Fas-mediated apoptosis of peripheral blood mononuclear cells in patients with hepatitis"C.Br J Haematol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Noriyasu Taya. Et al: "Fas-mediated apoptosis of perpheral blood mononuclear cells in patients with hepatitis"C. Br J Haematol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Noriyasu Taya, et al.: "Fas-mediated apoptosis of peripheral blood mononuclear cells in patients with hepatitis"C. Br J Haematol. (in press).

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi