| Project/Area Number |
10670941
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Nagoya University |
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Principal Investigator |
EMI Nobuhiko School of Medicine, Research Associate, 医学部, 助手 (30185144)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Akihiro School of Medicine, Medical Staff, 医学部, 医員
阿部 明弘 名古屋大学, 医学部, 医員
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Leukemia / PDGFR / Chromosome / CEV14 / Leukemia |
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| Research Abstract |
Chromosomal translocations involving band 5q31-35 occur in several hematologic disorders. A clone with a t(5;14)(q33;q32) translocation appeared at the relapse phase in a patient with acute myelogenous leukemia who exhibited a sole chromosomal translocation, t(7;11) at initial diagnosis. Following the appearance of this clone, the leukemia progressed with marked eosnophilia, and combination chemotherapy was ineffective. Southern blot analysis revealed a rearrangement of the PDGFRβ gene at 5q33 which was not observed at initial diagnosis. This translocation resulted in a chimeric transcript fusing the platelet derived growth factor β receptor gene on 5q33 with a novel gene, CEV14, located at 14q32. Expression of the 5' region of the PDGFRβ cDNA, upstream of the breakpoint, was not detected. However, the 3' region of PDGFRβ which was transcribed as part of the CEV14-PDGFRβ fusion gene was detected. The novel gene, CEV14, includes a leucine zipper motif and putative thyroid hormone receptor interacting domain and is expressed in a wide range of tissues. The expression of a CEV14-PDGFRβ fusion gene in association with aggressive leukemia progression suggests that this protein has oncogenic potential.
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