| Project/Area Number |
10670953
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | Kagawa Medical University |
|---|
Principal Investigator |
NAGAI Masami University Hospital, Kagawa Medical University Assistant Professor, 医学部・附属病院, 講師 (40180450)
|
|---|
| Project Period (FY) |
1998 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | Secondary leukemia / leukemogenesis / acetyltransferase / Histone / transcription / chromosome / ヒストンアセチル化酵素 / 染色体異常 / 細胞株 / MOZ / p300 / p33ING1 / p53 / NKcell / がん遺伝子 / がん抑制遺伝子 / 診断 / P300 / P53 / P33ING1 |
|---|
| Research Abstract |
The incidence of secondary, therapy-related leukemias has been increasing with the development of high-dose chemo-radiotherapy ; however, its molecular genetic mechanisms still remained unclear. We recently encountered a patient with 14-years-history of primary macroglobulinemia, who finally developed secondary, therapy-related monocytic leukemia. The G-banded and SKY analysis found that leukemic cells carried t (8 ; 22) (p11 ; q13) translocation. FISH and Southern blot analysis showed p300 and MOZ genes were involved in this translocation. RT-PCR analysis demonstrated fusion transcripts of these two genes, and the sequential analysis of the fusion product indicated that the product had two acetyl transferase domains. These results suggest aberrant expression of acetyl transferase might play an important role in the development of secondary leukemia.
|
