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Analysis of cell cycle modulating proteins in the process of polyploidization of megakaryocytes.

Research Project

Project/Area Number 10670970
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionTOKYO WOMEN'S MEDICAL UNIVERSITY

Principal Investigator

TERAMURA Masanao  Tokyo Women's Medical University, Department of Hematology, Lecturer, 医学部, 講師 (40188686)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Shoko  Tokyo Women's Medical University, Department of Hematology, Instructor, 医学部, 助手 (80256528)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsmegakaryocyte / polyploidization / cdc2 / cyclin B1 / NF-E2 / 多倍体化 / cyclin B1 / cdc 2
Research Abstract

Mechanism of polypoidization of megakaryocyte remain largely unclarified. To clarify them further, we used a human megakaryoblastic cell line, Meg-J, which showed prominent polyploidization and augmented platelet glycoprotein (GP) Ib expression after incubation with K252a (an indolocarbasole derivative). In the process of K252a-induced polyploidizaton, levels of both cdc2 and cyclin B1 were elevated transiently and subsequently decreased. This suggests that the polyploidization process in Meg-J cells is at least in part associated with a transient elevation and subsequent decrease in the expression of cdc2/cyclin B1 complex. Next, we analyzed the expression of the transcription factors and observed that the expression of NF-E2 p45, but not those of GATA-1, GATA-2, Tal-1/SCL, Evi-1, and MafK, was increased after TPO and K252a stimulation. Gel-shift assay confirmed the enhanced binding activity to the NF-E2 site. The abolishment of NF-E2 p45 with NF-E2 antisense oligomers inhibited TPO plus K252a-induced polyploidization. These findings suggest that NF-E2 p45 is essential for the polyploidization of megakaryocytic cells.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report

Research Products

(4 results)

All Other

All Publications (4 results)

  • [Publications] Kobayashi S, et al: "Transcription factor NF-E2 is esential for the polyploidization of a human megakaroblastic cell line, Meg-J."Biochem Biophys Res Commun. 247. 65-69 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwabe K, et al :: "K-252a-induced polyploidization and differentiation of a human megakaryocytic cell line, Meg-J : transient elevation and subsequent suppression of cyclin B1 and cdc2 expression in the process of polyploidization."Br J Haematol. 102. 812-819 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koyayashi S, et al.: "Transciption factor NF-E2 is esential for the polyploidization of a human megakaroblastic cell line, Meg-J."Biochem Biophys Res Commun. 247. 65-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwabe K, et al.: "K-252a-induced polyploidization and differentiation of a human megakaryocytic cell line, Meg-J : transient elevation and subsequent suppression of cyclin B1 and cdc2 expression in the process of polyploidization."Br J Haematol. 102. 812-819 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary

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Published: 1998-03-31   Modified: 2016-04-21  

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