| Project/Area Number |
10670983
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
|
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| Research Institution | AKITA UNIVERSITY |
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Principal Investigator |
TASHIMA Yohtalou School of Medicine, Akita University, Professor, 医学部, 教授 (30049796)
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| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | plCln / low osmolality / actin / E.Coli / pICln / 骨格筋 / E.Coli / F-actin / 低浸透圧 |
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| Research Abstract |
(1) E.Coli cells expressing mammalian plCln showed a significant increase in the resistance against a low environmental osmolality.
(2) Only 46-amino acid sequence included in the plCln molecule was enough for the acquired resistance with E.Coli cells.
(3) plCln in PPL-K1 cells (originated from pig kidneys) was moved and spread into cytosol from perinuclear region by the exposure of the cells to a low environmental osmolality.
(4) plCln in skeletal muscles was presented in a homologous form of hetero-oligomer with a constant length of actin-oligomer.
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