REGULATORY MECHANISM OF Cl-CHANNEL BY plCln.
| Project/Area Number |
10670983
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | AKITA UNIVERSITY |
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Principal Investigator |
TASHIMA Yohtalou School of Medicine, Akita University, Professor, 医学部, 教授 (30049796)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | plCln / low osmolality / actin / E.Coli / pICln / 骨格筋 / E.Coli / F-actin / 低浸透圧 |
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| Research Abstract |
(1) E.Coli cells expressing mammalian plCln showed a significant increase in the resistance against a low environmental osmolality.
(2) Only 46-amino acid sequence included in the plCln molecule was enough for the acquired resistance with E.Coli cells.
(3) plCln in PPL-K1 cells (originated from pig kidneys) was moved and spread into cytosol from perinuclear region by the exposure of the cells to a low environmental osmolality.
(4) plCln in skeletal muscles was presented in a homologous form of hetero-oligomer with a constant length of actin-oligomer.
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Report
(3 results)
Research Products
(11 results)
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[Publications] Li,Y., Tao,G-Z., Nagasawa, H., Tazawa, H., Kobayashi, A., Itoh, H., Tashima, Y.: "plCln and cytosolic F-action constitute a heteromeric complex with a constant molecuylar mass in rat skeletal muscles."J. Biochem.. 126. 643-649 (1999)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Publications] Ma,Y-Z., Tao, G-Z., Kobayashi, A., Itoh, H., Tashima, Y., Kojima, N., Senoo, H.: "Translocation of plCln in a pig kidney cell line, LLC-PK1, by low osmotic stress."Clin. Exp. Nephrol.. 3. 163-168 (1999)
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Publications] Li, Y., Tao, GZ, Nagasawa, H., Tazawa, H., Kobayashi, A., Itoh, H., Tashima, Y: "pICln and cytosolic F-actin constitute a heteromeric complex with a constant molecular mass in rat skeletal muscles"J. Biochem.. 126. 643-649 (1999)
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[Publications] Ma, YZ, Tao, GZ, Kobayashi, A., Itoh, H., Tashima, Y. Kojima, N. Senoo H: "Translocation of pIcln in a pig kidney cell line,LLC-PK1 by low osmotic stress"Clin Exp Nephrol.. 3. 163-168 (1999)
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