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Functional expression, intracellular translocation, structure-function relationship of water channels in the collecting duct.

Research Project

Project/Area Number 10670986
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUWAHAWA Michio  Tokyo Medical and Dental University, School of Medicine, Lecturer, 医学部, 講師 (60221230)

Co-Investigator(Kenkyū-buntansha) SASAKI Sei  Tokyo Medical and Dental University, School of Medicine, Associate Professor, 医学部, 助教授 (60170677)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsglucocorticoid / cell cycle / mesangial cell / nitric oxide / AQP2 / molecular biology / C. elegans / 水チャネル
Research Abstract

1. We examined the intracellular mechanism of glucocorticoids action using mesangial cells and an experimental model of anti-GBM nephritis. Our results suggest that glucocorticoids stimulate p21CIP1 gene promoter activity and cause cell cycle arrest. This action may be associated with the effectiveness of glucocorticoids in the treatment of glomerulonephritis.
2. We investigated the intracellular signal transduction pathways to clarify the pathogenesis of glomerulonephritis. New findings are ; 1) inducible nitric oxide synthase is induced in the absence of active NF-kB, 2) p27Kip1 and p21CIP1, cell cycle inhibitors, cause cell hypertrophy, and 3) TGF-β-activating kinase-1 inhibits cell cycle and expression of cyclin D1 and A.
3. We reported a case of autosomal dominant nephrogenic diabetes insipidus that is cased by a deletion of seven bases in the AQP2 gene (809del 7). We found that this mutation causes an inhibition of transcription or a trafficking defect.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Ishibashi K et al.: "Cloning and functional expression of a new aquaporin abundantly expressed in the peripheral leukooyte perneable to water and area, but not to glycerl"Biochem. Biophys.Res. Commun.. 244. 268-274 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Goji K et al.: "Novel mutations in the aquaporin-2 gene in femal siblings with nephrogenic diabetes insipidus"J. Clin. Endocrinol. Metab.. 83. 3205-3209 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terada Y. et al.: "Lovastatin inhibits mesangial cell proliferation via p27Kip1"J. Am. Soc. Nephrol.. 9. 2235-2243 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koyama N et al.: "Cloning and functional expression of human aquaporin 8 CDNA and anolysis of its gene"Genomics. 54. 169-172 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuwahara M et al.: "A water channel of the nematode C. elegans and its implications for channel selectivity of MIP proteins"Am. J. Physiol.. 275. C1459-C1464 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakashima O. et al.: "Inducible nitric oxide synthase can be induced in the absence of active NF-KB in rat mesangicol cells"J. Am. Soc. Nephrol.. 10. 721-729 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ko SBH. et al.: "Cloning and functional expression of rAQPL"Biochem. Mol. Biol. Int.. 47. 309-318 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terada Y. et al.: "Cell cycle inhibitors (p27Kip1 and p21CIP1) cause hypertrophy in LLC-PK1 cells"Kidney Int.. 56. 494-501 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terada Y. et al.: "TGF-β-activating kinase-1 inhibits cell cycle and expression of cyclin D1 and A in LLC-PK1 cells"Kidney Int.. 56. 1378-1390 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuwahara M: "Transmembrane helix 5 is critical for the high water permeability of aquaporin"Biochemistry. 38. 16340-16346 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishibashi K et al.: "Cloning and functional expression of a new aqaporin abundantly expressed in the peripheral leukocyte permeable to water and urea, but not to glycerol."Biochem. Biophys. Res. Commun.. 244. 268-274 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Goji K et al.: "Novel mutations in the aquaporin-2 gene in female siblings with nephrogenic diabetes insipidus : Evidence of disrupted water channel function."J. Clin. Endocrinol. Metab.. 83. 3205-3209 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terada Y. et al.: "Lovastatin inhibits mesangial cell proliferation via p27Kip1."J. Am. Soc. Nephrol.. 9. 2235-2243 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koyama N. et al.: "Cloning and functional expression of human aquaporin 8 cDNA and analysis of its gene."Genomics. 54. 169-172 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuwahara M. et al.: "A water channel of the nematode C. elegans and its implications for channel selectivity of MIP proteins."Am. J. Physiol. 275. C1459-C1464 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakashima O. et al.: "Inducible nitric oxide synthase can be induced in the absence of active NF-kB in rat mesangial cells: Involvement of Janus kinase 2 signaling pathway."J. Am. Soc. Nephrol.. 10. 721-729 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ko S. B. H. et al.: "Cloning and functional expression of rAQP9L : a new member of aquaporin family from rat liver."Biochem. Mol. Biol. Int.. 47. 309-318 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terada Y. et al.: "Cell cycle inhibitors (p27Kip1 and p21CIP1) cause hypertrophy in LLC-PK1 cells."Kidney Int.. 56. 494-501 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terada Y. et al.: "TGF-β-activating kinase-1 inhibits cell cycle and expression of cyclin D1 and A in LLC-PK1 cells."Kideny Int.. 56. 1378-1390 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kurwahara M. et al.: "Transmembrane helix 5 is critical for the high water permeability of aquaporin."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakashima O. et al: "Inducible nitrix oxide synthase can be induced in the absense of active NF-KB in rat mesangical cells"J. Am. Soc. Nephrol. 10. 721-729 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ko SBH et al: "Cloning and functional expression of rAQP9L"Biochem. Mol. Biol. Int. 47. 309-318 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Terada Y et al: "Cell cycle inhibitors (p27kip 1 and P21cip 1) cause hypertrophy in LLC-PK1 cells"Kidney Int.. 56. 494-501 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Teuada Y et al.: "TGF-β-activating kinase-1 inhibits cell cycle and expression of cyclin D1 and A in LLC-PK1 cells"Kidney Int.. 56. 1378-1390 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kuwahara. M et al: "Transmembrane helix 5 is critical for the highwater permeatility of aquaporin"Biochemistry. 38. 16340-16346 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Ishibashi: "Cloning and functional expression of a new aquaporin(AQP9)abundantly expressed in the peripheral laukoeytes permeable to water and urea, but not glycerol" Biochem. Biophys. Res. Commun.244. 268-274 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K. Goji: "Novel mutations in aquaporin-2 gene in femals siblings with nephrogenic diabetes insipidus : Evidence of disrupted water channel function" J. Clin. Endcrin. Metab.83. 3205-3209 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y. Terada: "Lovastatin inhibits mesangial all proliferetion via p27^<Kip1>" J. Am. Soc. Nephrol.9. 2235-2243 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N. Koyama: "Cloning and functional expression of human aquaporin8 cDNA and analysis of its gene" Genomics. 54. 169-172 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M. Kuwahara: "A water channel of the nematode C elegans and its implication for channel selectivity of MIP-proteins" Am. J. Physiol.275. C1459-C1464 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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