| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Research Abstract |
The pathophysiological role and the critical epitope of Thy-l.1 molecule for induction of mesangial cell dysfunctionremains unknown. Immunofluorescense and immuno-electron microscopic studise showed that the precise subcellularlocaclization of the specific epitopes of Thy-l.1 molecule, which were recognized by two kinds of monoclonal antibodies, 1-22-3 and OX-7, were quite different. An epitope recognized by 1-22-3 was concentrated specifically on the mesangial cell surfaces facing the neighboring endothelial cells. Wheares OX-7 bound to mesangial cell surfacesand extracellular parts in diffuse pattern independent of contact with endothelial cells. Incubation of the mesangialcells with 1-22-3 resulted in increased IP3 level, sigificantly greater than with OX-7. When injected in rats, 1-22-3 produced much urinary protein excreation than OX-7. We believe that criticalepitope detected by 1-22-3 in this study plays an important role in mesangial function and injury. This molecule is presented on the rat mesangial cells, but there was no study whether these molecules are presented on the human mesangial cells. In the present study, 1) we estimate the epitope, which is recognized by monoclonal antibody 1-22-3, was present on 1-66 amino acid sequence of thy-1, 2) we showed that the thy-1 like molecule is presented on the human mesangial cells using RT-PCR and immunohistochemistry.
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