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Platelet-activating factor acetylhydrolase gene mutation in Japanese nephrotic children.

Research Project

Project/Area Number 10670997
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionKobe University

Principal Investigator

YOSHIKAWA Norishige  Kobe University, School of Medicine, Professor, 医学部, 教授 (10158412)

Co-Investigator(Kenkyū-buntansha) IIJIMA Kazumoto  Kobe University, School of Medicine, Assistant, 医学部, 助手 (00240854)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsJapanese children / steroid-responsive nephrotic syndrome / relapse / mutations / Platelet-activating factor / Platelet-activating factor acetylhydrolase / gene mutation
Research Abstract

Platelet-activating factor (PAF) may be involved in the pathogenesis of steroid-responsive nephrotic syndrome (SRNS). PAF is degraded to inactive products by PAF acetylhydrolase. We have investigated whether PAF acetylhydrolase gene mutation (G994T) is involved in SRNS in Japanese children. We identified a point mutation in the PAF acetylhydrolase gene (G to T transversion at position 994) using the polymerase chain reaction in 101 Japanese children with SRNS and 100 healthy Japanese. We then determined the relationship between the PAF acetylhydrolase G994T gene mutation and relapse of nephrotic syndrome. There was no difference in the genotype and allele frequencies between patients with SRNS and normal controls. The mean number of relapses during the first year after onset was significantly higher in the 26 patients who were heterozygous for the mutant allele (GT) than in 75 wild-type homozygotes (GG) (2.61±1.98 vs. 1.33±1.35 ; p=0.0019). Patients with the mutant (GT) genotype relapsed more often than patients with the wild-type (GG) genotype (xィイD12ィエD1)=15.8, p=0.0033). We conclude that analysis of the PAF acetylhydrolase gene mutation at position 994 in Japanese children with SRNS allows the identification of patients who are more likely to have disease relapse.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Nakanishi, K., Yoshikawa, N. et al.: "α5 (IV) collagen chain expression in skin and disease severity in women with X-linked Alport syndrome"J. Am. Soc. Nephrol.. 9. 1433-1440 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Noguchi, K., Yoshikawa, N. et al.: "Activated mesangial cells produce vascular permeability factor in early-stage mesangial proliferative glomerulonephritis"J. Am. Soc. Nephrol.. 9. 1815-1825 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Xu, H., Yoshikawa, N. et al.: "Platelet-activating factor acetylhydrolase gene mutation in Japanese nephrotic children"Kidney Int.. 54. 1867-1871 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka, R., Yoshikawa, N. et al.: "Role of platelet-activating factor acetylhydrolase gene mutation in Japanese childhood immunoglobulin A nephropathy"Am. J. Kidney Dis.. 34. 289-295 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue, Y., Yoshikawa, N. et al.: "Detection of mutation in the COL4A5 gene in over 90% of male patients with X-linked Alport syndrome by reverse transcription-polymerase chain reaction and direct sequence analysis"Am. J. Kidney Dis.. 34. 854-862 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Xu, H., Yoshikawa, N. et al.: "Platelet-activating factor acetylhydrolase mutation in Japanese children with HUS"Am. J. Kidney Dis.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Xu H, Iijima K, Shiozawa S, Tanaka R, Inoue Y, Shirakawa T, Nishiyama K, Nakamura H and Yoshikawa N: "Platelet-activating factor acetylhydrolase gene mutation in Japanese nephrotic children"Kidney Int. 54. 1867-1871 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue Y, Iijima Y, Nakamura H and Yoshikawa N: "Benefit and chronic nephrotoxicity of 2-year cyclosporin treatment in children with steroid-dependent minimal change nephrotic syndrome : A prospective study"Pediatr Nephrol. 13. 33-38 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshikawa N, Iijima K and Ito H: "Cyclosporin treatment in children with steroid-dependent nephrotic syndrome"Clin Exp Nephrol. 10. S27-33 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka R, Xu H, Iijima K, Shiozawa S, Shirakawa T, Nakamura H and Yoshikawa N: "Role of platelet-activating factor acetylhydrolase gene mutation in Japanese childhood immunoglobulin A nephropathy"Am J Kidney Dis. 34. 289-295 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Xu H, Iijima K, Shiozawa S, Shirakawa T, Nakamura H, Yoshikawa N: "Platelet-activating factor acetylhydrolase mutation in Japanese children with HUS"Am J Kidney Dis. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakanishi K,Yoshikawa N et al.: "α5(IV)collagen chain expression in skin and disease severity in women with X-linked Alport syndrome"J Am Soc Nephrol. 9. 1433-1440 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noguchi K,Yoshikawa N et al.: "Activated mesangial cells produce vascular permeability factor in early-stage mesangial proliferative glomerulonephritis"J Am Soc Nephrol. 9. 1815-1825 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Xu H,Yoshikawa N et al.: "Platelet-activating factor acetylhydrolase gene mutation in Japanese nephrotic children"Kidney Int. 54. 1867-1871 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka R,Yoshikawa N et al.: "Role of platelet-activating factor acetylhudrolase gene mutation in Japanese childhood immunoglobulin A nephropathy"Am J Kidney Dis. 34. 289-295 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Inoue Y,Yoshikawa N et al.: "Detection of mutations in the COL4A5 gene in over 90% of male patients with X-linked Alport syndrome by reverse transcription-polymerase chain reaction and direct sequence analysis"Am J Kidney Dis. 34. 854-862 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Xu H,Yoshikawa N et al.: "Plafelet-activating factor acetylhydrolase mutation in Japanese children wirh HUS."Am J Kidney Dis. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshikawa N.他: "Platelet-activating factor acetylhydrolase gene mutation in Japanese nephrotion children" Kidney International. 54. 1867-1871 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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