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Cloning of Ca permeable ion channel in he kidney.

Research Project

Project/Area Number 10671002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionJichi Medical School

Principal Investigator

SUZUKI Makoto  JICHI Medical School, ACCOCIATED PROFESSOR, 医学部, 助教授 (10196868)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsIonchannel / Ca / Na / 張力感受性
Research Abstract

To investigate molecules contributing Ca transport in the kidney, Isolation and in vitro expression of cDNAs was designed to be the subject of this study. By homology of Trp families, we isolated 4 different families; spliced variants of Trps, valiant of vanilloid receptors and its family, and Epithelial Ca channel family. Variant of vanilloid receptor has been unique mechanosensitive channel where stretch diminished the activity of this channel. Trp variant was further unique in expression, since original clone has not been expressed the Xenopus oocytes system we used. Ca permeable channels are compatible with our aims, although the cloning reported by others independently. We could express two clones of this family and find important channels in Ca homeostasis in the body.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report

Research Products

(7 results)

All Other

All Publications (7 results)

  • [Publications] H. Suzuki et al: "Cloning of a stretch-inhibitable nonselective cation channel"The J. Biol. Chem.. 247(10). 6330-6335 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Miyosh et al: "Expression of an mNSC1 in mammalian cells"Biochem. Biophys Res. Comm.. 265(1). 13-17 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,M., Satoh,J., Kutsuwada,K., Ohki,G., Imai,M.: "Cloning of a stretch-inhibitable nonselective cation channel."The J. Biol. Chem.. 247(10). 6330-6333 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyoshi,T., Ooki G., Murata,M., Hayakawa,H., Tomita, K., Imai,M., Suzuki,M.: "Expression of an mNSC1 in mammalian cells."Biochem. Biophys Res. Comm.. 265(1). 13-17 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Suzuki et al.: "Cloming of a stretch-muetivated cation channel"JBC. 247. 6330-6335 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. MIYOSHI et al.: "Expression of mNSC in Mamnalicm cells"BBRC. 265. 13-17 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.SOZUKI: "Cloning of a stretch-inhibitable nonselective catron channel" J.Biol.Chem. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-03-31   Modified: 2016-04-21  

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