• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Mechanism of Glucagon for Suppression of Aldolase B Gene Transcription

Research Project

Project/Area Number 10671055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

KAZUMAKI Takejiro (1999)  School of Medicine, Yamagata Univ. Associate Professor, 医学部, 助教授 (70211208)

石川 喜一 (1998)  山形大学, 医学部, 教授 (40018312)

Co-Investigator(Kenkyū-buntansha) IUCHI Yoshihito  School of Medicine, Yamagata Univ. Instructor, 医学部, 助手 (60272069)
葛巻 丈二朗  山形大学, 医学部, 助教授 (70211208)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsAldolase / Gene Expression / Glucagon / Hepatocytes / Glucose Metabolism
Research Abstract

Transcription of the aldolase B gene, AldB, in the liver is regulated by hormones such as insulin and glucagon. We investigated the molecular mechanism of glucagon for suppression of aldolase B gene expression since glucagon is a potent suppressor for aldolase B gene transcription. In this research project, we clarified the signal transduction pathway triggered by glucagon and identified the glucagon-responsive element and insulin-responsive region in aldolase B gene promoter. Glucagon binds to the specific receptor located on the surface of hepatocytes. We examined the signal transduction pathway using H-7 (protein kinase A inhibitor), forskolin and dbcAMP (cAMP agonist) in rat primary cultured hepatocytes. By the experiments, we concluded that glucagon suppresses the aldolase B gene expression through the cAMP/protein kinase A pathway. To characterize the elements which are responsive to glucagon in the upstream region of AldB, plasmids carrying various length of the upstream region … More of this gene were constructed and transfected to primary cultured rat hepatocytes. The transcription activities of the mutants containing the sequences between -764 and +25 bp were suppressed by glucagon. Within this region, the sequence element similar to the cAMP-responsive element (CRE) was found between -89 and -82bp (designated CRE-89). The deletion of the CRE-89 element diminished the responsiveness to glucagon. A gel retardation assay showed that the nuclear factors bind to the CRE-89 element. These results suggest that the CRE-89 element in the promoter region is prerequisite for suppression of the gene by glucagon in hepatocytes. On the other hand, insulin enhanced the transcription activity of aldolase B gene. The transcription activities of the mutants containing the sequences between -228 and -85 bp were enhanced by insulin. Within this region, the known insulin-responsive elements (IRE) was not found. Novel IRE is suggested to involve in the activation of aldolase B gene expression Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Jun-itsu Ito,Takejiro Kuzumaki,et al.: "Hormonal regulation of aldolase B gene expression in rat primary cultured hepatocvtes"Archives of Biochemistry and Biophysics. 350(2). 291-297 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yumiko Takano,Yoshihito Iuchi,et al.: "Characterization of the responsive elements to hormones in rat aldolase B gene"Archives of Biochemistry and Biophysics. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 石川喜一、葛巻丈二朗: "ホルモンによるB型アルドラーゼ遺伝子の転写制御"生化学. 71(10). 1213-1223 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 石川喜一: "B型アルドラーゼ遺伝子の転写調節機構とホルモンによる制御"山形大学紀要(医学). (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jun-itsu Ito, Takejiro Kuzumaki, Kaoru Otsu, Yoshihito Iuchi, and Kiichi Ishikawa: "Hormonal regulation of aldolase B gene expression in rat primary cultured hepatocytes"Archives of Biochemistry and Biophysics. 350(2). 291-297 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yumiko Takano, Yoshihito Iuchi, Jun-istu Ito, Kaoru Otsu, and Kiichi Ishikawa: "Characterization of the responsive elements to hormones in the rat aldolase B gene"Archives of Biochemistry and Biophysics. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kiichi Ishikawa and Takejiro Kuzumaki: "Transcriptional regulation of Aldolase B gene by hormones (Japanese)"Seikagaku. 71(10). 1213-1223 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kiichi Ishikawa: "Hormonal regulation of Aldolase B gene expression (Japanese)"Yamagata Medical Journal. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yumiko Takano,Yoshihito Iuchi,et al.: "Characterization of the responsive elements to hormones in rat aldolase B gene"Archives of Biochemistry and Biophysics. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 石川喜一、葛巻丈二郎: "ホルモンによるB型アルドラーゼ遺伝子の転写抑制"生化学. 71(10). 1213-1223 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 石川喜一: "B型アルドラーゼ遺伝子の転写調節機構とホルモンによる制御"山形大学紀要(医学). (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Jun-itsu Ito: "Hormonal Regulation of Aldolase B Gene Expression in Rat Primary Cultured Hepatocytes" Archives' of Biochemistry and Biophysics. 350(2). 291-297 (1998)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi