| Project/Area Number |
10671065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Osaka University |
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Principal Investigator |
INUI Yoshiaki Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (00294067)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Shinji Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (30243223)
KAWATA Sumio Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (90183285)
MATSUZAWA Yuji Osaka University Graduate School of Medicine, Professor, 医学系研究科, 教授 (70116101)
KOHNO Kenji Nara Institute of Science and Technology Research and Education Center for Genetic Information, Professor, 遺伝子教育研究センター, 教授 (50142005)
KAYANOKI Yoshiro Osaka University Medical School, Medical Staff, 医学部・附属病院, 医員
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | HB-EGF / atherosclerosis / smooth muscle cell / transform / in vitro model of artery wall / visceral fat / ノックアウトマウス / 増殖因子 / in vivo / 治療 |
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| Research Abstract |
Balloon catheter injury of arteries induces migration and proliferation of smooth muscle cells (SMCs), with subsequent neointimal formation. It is important for the patients to suppress the proliferation and migration of SMCs in the process of neointimal accumulation induced by arterial injury. In this study, we developed the in vitro three dimension model of artery wall and investigated the effect of heparin-binding epidermal growth factor-like growth factor (HB-EGF), which is a potent SMC stimulator. In atherosclerotic plaque, HB-EGF protein was strongly positive in the intima. In the injured arteries by ballon catheter, northern blot analysis showed that the transcript levels of HB-EGF increased rapidly within 2 hours after injury. The addition of HB-EGF protein induced the expression of other growth factors. These data suggest that HB-EGF may play an important role of the proliferation and migration of SMCs in the process of neointimal accumulation induced by arterial injury, probably in an autocrine, paracrine, and/or juxtacrine manner. The expression of HB-EGF gene and serum concentration of HB-EGF protein were examined in the fat tissue, demonstrated that the gene expression was increased in the visceral fat tissue and that the serum concentraion was correlated with atherosclerosis.
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