Mechanism of PI 3-kinase activation during adipocyte differentiation
| Project/Area Number |
10671073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kobe University |
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Principal Investigator |
OGAWA Wataru Kobe University School of Medicine, Second Department of Internal Medicine, Assistant Professor, 医学部, 助手 (40294219)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | PI 3-kinase / adipocytes / differentiation / signal transduction / insulin action / インスリン作用 / PI3-キナーゼ / チロシンキナーゼ |
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| Research Abstract |
Differentiation of adipocytes is an important aspect of energy homeostasis as well as insulin sensitivity. In this research project, we have found that PI 3-kinase is activated during adpocyte differentiation by associating with tyrosine-phosphorylated IRS-1. We have now trying to identify a tyrosine kinase that phosphorylates IRS-1 during adpipocyte differentiation. We also found that PI 3-kinase promotes adipogenesis at the step of translation of adipocyte-specific proteins and that phosphorylation of 4E-BP1 induced by a serine threonin kinase Akt is an important step in PI 3-kinase-mediated translational initiation. Intracellular concentration of cAMP is critical for adipogenesis as well as insulin action in adipocytes. We demonstrated that phosphodiesterase (PDE) 3B plays a major role in regulation of cAMP. Furthermore, Akt mediates activation of PDE 3B by directly phosphorylating the enzyme.
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Report
(3 results)
Research Products
(11 results)
