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Anti-angiogenic drug AGM1470 suppresses smooth muscle cell migration induced by endothelial PDGF.

Research Project

Project/Area Number 10671102
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionTHE UNIV. OF TOKYO

Principal Investigator

HOSOI Yutaka (1999)  The University of Tokyo, Faculty of Medicine, Assistant., 医学部・附属病院, 助手 (40311625)

小見山 高士 (1998)  東京大学, 医学部・附属病院, 助手 (10292947)

Co-Investigator(Kenkyū-buntansha) HATAKEYAMA T.  The University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 助手 (60291324)
MIYATA T.  The University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部・附属病院, 講師 (70190791)
SHIGEMATSU Hiroshi  The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 助教授 (40134556)
重松 邦広  東京大学, 医学部・附属病院, 助手
SHIGEMATSU K.  The University of Tokyo, Faculty of Medicine, Assistant
細井 温  東京大学, 医学部・附属病院, 助手
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsNeovascularization / Hyperplasia of anast / Endothelium, Vascular / Smooth muscle cell / Shear stress
Research Abstract

OBJECTIVES: To examine the effects of the anti-angiogenic drug AGM1470 on smooth muscle cell (SMC) migration activity stimulated by endothelial cell (EC-derived mitogen. MATERIALS AND METHODS: Study 1 ; EC's were cultured under pulsatile flow using MCDB151 medium. From the supernatant of these EC dishes we devised two types of conditioned medium; anti-PDGF(+) containing 10 micrograms/ml anti-PDGF antibody, and anti-PDGF(-) containing no antibody. SMC's were cultured using both media. Study 2 ; EC's were cultured under the same conditions using both types of medium; MCDB151 medium containing 10 ng/ml AGM1470, and MCDB151 medium alone. After the AGM1470 concentration had been adjusted to 10 ng/ml, SMC's were cultured using each medium ; AGM-exposed EC and AGM-non-exposed EC. SMC colony spreading distances were measured as an index of mitogenic activity for 4 days. RESULTS: Study 1 ; the anti-PDGF(-) group showed an apparently greater spreading distance than the anti-PDGF(+) group. Study 2 ; the AGM-non-exposed EC group showed a significantly greater spreading distance than the AGM-exposed EC group. However, MTT assay revealed no differences in proliferation between the two groups. CONCLUSION: AGM1470 suppresses the EC production of this PDGF-like mitogen as well as SMC migration activity.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Shigematsu K, et al.: "Anti-angiogenic drug AGM-1470 suppress smooth muscle cell migration induced by endothelial PDGF"European Journal of Vascular Endovascular Surgery. 14. 290-298 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigematsu K, et al.: "Inhibitory effects of anti-angiogenic agent AGM-1470 on colon cancer cells in vitro study of mitogens prduced by cancer cells"Microcirculation Annual. 13. 93-94 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigematsu H.: "Diabetes and peripheral arterial occlusion"Asian Medical Journal. 41(5). 253-280 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 重松 邦広: "人工血管吻合部内膜肥厚に対する血流の影響"運動療法と物理療法. 9(2). 104-106 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigematsu K, Yasuhara H, Shigematsu H, et al.: "Anti-angiogenic drug AGM-1470 suppresses smooth muscle cell migration induced by endothelial PDGF."European Journal of Vascular and Endovascular Surgery. 14. 290-298 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigematsu K, Yasuhara H, Shigematsu H, et al.: "Inhibitory effects of anti-angiogenic agent AGM-1470 on colon cancer cells - in vitro study of mitogens produced by cancer cells."Microcirculation Annual. 13. 93-94 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigematsu H.: "Diabetes and peripheral arterial occlusion"Asian Medical Journal. 41(5). 253-260 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigematsu K.: "The effect of blood flow on anastmotic intimal hyperplasia"kinesiology and physical therapy. 9(2). 104-106 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 重松 宏: "特集によせて"血栓と循環. 6(1). 9 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 重松 宏: "糖尿病と末梢動脈閉塞"日本医師会雑誌. 118(2). 215-220 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] 重松 宏: "末梢血管障害の病態と最近の話題"Pharma Media. 15(12). 85-89 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shigematsu H.: "Diabetes and peripheral arterial occlusion"Asian Medical Journal. 41(5). 253-260 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 重松 邦広: "人工血管吻合部内膜肥厚に対する血流の影響"運動療法と物理療法. 9(2). 104-106 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 細井 温: "血管疾患の無侵襲診断法"医歯薬出版. 6 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 重松 宏: "特集によせて" 血栓と循環. 6(1). 9 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 重松 宏: "糖尿病と末梢動脈閉塞" 日本医師会雑誌. 118(2). 215-320 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 重松 宏: "末梢血管障害の病態と最近の話題" Pharma Medica. 15(12). 85-89 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shigematsu H.: "Diabetes and peripheral arterial occlusion" Asian Medical Journal. 41(5). 253-260 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 重松 邦広: "人工血管吻合部内膜肥厚に対する血流の影響" 運動療法と物理療法. 9(2). 104-106 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 細井 温: "血管疾患の無侵襲診断法" 医歯薬出版, 6 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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