| Project/Area Number |
10671120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Ehime University |
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Principal Investigator |
HONDA Kazuo Department of Surgery, Ehime University, Associate professor, 医学部, 助教授 (00209321)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Liver / gene transfer / myoglobin / mouse / apoptosis / adenovirus |
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| Research Abstract |
Hemoglobin gene was transferred to mouse liver to estimate the protective effect of newly expressed hemoglobin from ischemia-reperfusion injury. The adenovirus vector containing myoglobin gene was administered through the penial vein of C57BL mice and the liver samples were analyzed by RT-PCR and immunohistochemistry at 3 days after gene transfer. RT-PCR revealed the expression of mRNA of the myoglobin gene in the liver. Immunohistochemistry showed positive staining of myoglobin protein in the hepatocytes. The hepatoduodenal ligaments of the gene-transferred and control mice were clamped for 30 minutes for total hepatic ischemia and the liver samples were collected at 24 hours after reperfusion. DNA was extracted and subjected to electrophoresis on agarose gels. The DNA ladder was detected in the control mice while the DNA from the gene-transferred mice showed no ladder. TUNEL staining revealed positive staining in the control mice while the gene-transferred mice showed a few TUNEL positive cells. Serum ALT increased in the control mice but it remained normal in the gene-transferred mice. Gene transfer of hemoglobin was effective in protecting the liver form apoptosis after ischemia-reperfusion injury.
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