| Project/Area Number |
10671129
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Jichi Medical School |
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Principal Investigator |
KOBAYASHI Eiji Jichi Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00245044)
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| Co-Investigator(Kenkyū-buntansha) |
HAKAMATA Yoji Jichi Medical School, Faculty of Medicine, Lecturer, 医学部, 講師 (00218380)
MURAKAMI Takashi Jichi Medical School, Faculty of Medicine, Assistant, 医学部, 助手 (00326852)
YOSHIDA Shigeto Jichi Medical School, Faculty of Medicine, Lecturer, 医学部, 講師 (10296121)
HOSOYA Yoshinori Jichi Medical School, Faculty of Medicine, Assistant, 医学部, 講師 (30275698)
FUJIMURA Akio Jichi Medical School, Faculty of Medicine, Professor, 医学部, 教授 (90156901)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | gene therapy / Gene Gun / local therapy / solid tumor therapy |
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| Research Abstract |
Using a hand-held gene gun which accelerates DNA-coated microparticles, we examined the effects of interleukin-12 (IL-12) gene transfection on several models of rat solid cancer. AH130 cells were injected into portal vein and ileocecal mesenterium lymph node of Donryu rats, as a liver and lymph node metastatic model, respectively. Yoshida sarcoma cells were injected into the axillar skin lesion. Total amounts of 6 μg of IL-12 DNA coated gold particles were transferred into the liver with metastatic tumors by pressurized helium gas of 300 psi (1 psi=6,890Pa) on day 14 and 16 after the tumor inoculation. Four mg of IL-12 DNA was given into the lymph node metastatic tumor on day 7 and 9 after tumor cell injection skin tumor was bombarded by IL-12 with TNF- α. The corresponding amounts of β -galactosidase were given by the identical route as a control for each group. Compared with the control group, IL-12 gene therapy was statistically effective for the inhibition of tumor growth and for survival rates in the liver metastatic model. A few beneficial effect of IL-12 transduction were observed in the lymph node metastatic model. Skin tumor growth was significantly suppressed by combination of IL-12 with TNF- α. From the standpoint of surgery or surgeons, this method has potential as a new strategy for cancer, especially as an intraoperative gene therapy.
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