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Adenovirus-mediated anti-apoptotic gene (bcl-2) transfer in the transplant pancreas

Research Project

Project/Area Number 10671158
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionTokyo University

Principal Investigator

MAKUUCHI Masatoshi (1999)  University of Tokyo, Faculty of Medicine, Professor, 医学部・附属病院, 教授 (60114641)

青柳 信嘉 (1998)  東京大学, 医学部・附属病院, 助手 (90292903)

Co-Investigator(Kenkyū-buntansha) SHIONOIRI Futoshi  University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 医員
MIURA Yasuaki  University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 医員
ABE Hideki  University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 医員
ISHIBASHI Shun  University of Tokyo, Faculty of Medicine, Assistant Prof., 医学部・附属病院, 助手 (90212919)
HARADA Kenji  University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 医員
山田 信博  東京大学, 医学部・附属病院, 助教授 (40200729)
幕内 雅敏  東京大学, 医学部・附属病院, 教授 (60114641)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordspancreas / transplantation / insulin / adenovirus / Bcl2 (anti-apoptotic gene) / アデノウィルス / Bcl_2 / 拒絶反応 / 膵臓移植
Research Abstract

Background. Hyperinsulinemia, which is usually related to insulin resistance, is considered to be an important risk factor for coronary artery disease. The present study examines the influence of portal delivery of endogenous insulin after pancreas transplantation on plasma lipid metabolism, as compared to systemic delivery of insulin.
Method. Pancreas was transplanted heterotopically in normal rats by anastomosis of the donor portal vein to the recipient portal vein (PPTx) or inferior vena cava (CPTx) as an in vivo model of endogenous hyperinsulinemia.
Results. The mean value of plasma insulin concentration of CPTx and PPTx rats was 149% and 165% that of control rats, whereas the plasma glucose concentration of CPTx and PPTx rats did not differ significantly from that of control rats. Plasma triglyceride (TG) concentrations were significantly lower in both PPTx and CPTx rats than control rats. During the intravenous glucose tolerance test, the area under the insulin concentration curves (AUC) of CPTx and PPTx rats was 204% and 215% that of control rats, and they were correlated negatively with plasma TG concentrations. Plasma cholesterol and TG concentrations were significantly lower in PPTx rats than in control and CPTx rats.
Conclusions. Chronic hyperinsulinemia has a dose-dependent inhibitory effect on the regulation of plasma TG concentrations in rats with a transplanted pancreas. Significant lower lipid levels in PPTx rats than in CPTx rats suggest that portal delivery of insulin from the transplanted pancreas is relatively preventive for the atherosclerotic process as compared to systemic delivery of insulin.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 原田 賢治: "マクロファージのアポトーシスと動脈硬化"内分泌・糖尿病科. 9. 46-53 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideaki Abe, et al.: "Chronic inhibitory effect of insulin in rats with transplantation pancreas"Transplantation. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideki Abe, et al.: "Chronic inhibitory effect of insulin in rats with transplanted pancreas."Transplantation. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harada K, Ishibashi S, Yamada N.: "Association of tyrosine-phosphorylated 39 kD and 29 kD proteins with apoptotic cell death induced by monocyte/macrophage colony stimulating factor deprivation."American heart association 71ィイD1stィエD1 Scientific Sessions. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Abe H. et al.: "Chromic inhibitory effect of insulin on plasma lipid concentration in rats with transplanted pancreas"Transplantation. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 原田賢治: "マクロファージのアポトーシスと動脈硬化"内分泌・糖尿病科. 9(1). 46-53 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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