| Project/Area Number |
10671161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WATANABE Toshiakil The Univ. of Tokyo, Dept. of Surg., Associate professor, 医学部・附属病院, 助教授 (80210920)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Keiji The Univ. of Tokyo, Dept, of Surg., Assistant professor, 医学部・附属病院, 助手 (90302728)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Ulcerative colitis / Cancer / Dysplasia / p53 / adenoma / Microsatellite instability / P53 |
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| Research Abstract |
Genetic alterations in colorectal cancer and dysplasia (DS) in patients with ulcerative colitis (UC) were examined. Losses of heterozygosity (LOH) in chromosome 5q, 17p, 8p, 18q, 22p and microsatellite instability (MSI) were examined. LOH was observed in 36%(4/11)(5q), 67%(8/12)(17p), 59%(10/17)(8p), 0%(0/3)(18q) and 0%(0/3)(22p), which indicated the importance of the p53 gene in the development of colorectal tumors in UC. No lesions showed MSI. In an immunohistochemical analysis, the over expression of p53 was observed in 89% of invasive cancers, 70% of high-grade dysplasia, 57% of low-grade dysplasia and 0% of adenoma. This showed the effectiveness of immunohistochemical analysis for different diagnosis of adenoma and dysplasia in a clinical setting.
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