| Project/Area Number |
10671172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
ANDO Hisami Nagoya University, School of Medicine, Professor, 医学部, 教授 (60184321)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUNO Shinsuke School of Medicine, Medical Staff, 医学部, 医員
KANEKO Kenitirou School of Medicine, Medical Staff, 医学部, 医員
水野 敬輔 名古屋大学, 医学部, 医員
伊藤 不二男 名古屋大学, 医学部, 医員
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | choledochal cyst / pancreaticobiliary maljunction / whole-mount / enteric nerve system / whole mount / Whole mount |
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| Research Abstract |
1. The aim of this study is to elucidate the pathophysiological mechanism of bile duct dilatation which is characteristic of the choledochal cyst.
2. This study analyzed the bile duct walls of patients with choledochal cyst as to whether the nerve fibers and nerve cells in the wall are abnormal or not. Bile ducts obtained from eight patients with choledochal cyst and three normal bile ducts without dilatation were studied. These bile ducts were fixed in Zamboni solution. Whole-mount preparations displaying the myenteric plexus running beneath the outer surface of the bile duct were made by removing the mucosa, submucosa, fibromuscular layers under a stereomicroscope. Whole-mount preparations were immersed in 0.3% Triton X-phosphate buffer solution for 2 to 3 days. They were immunohistochemically stained using the streptavidin-diotin-peroxidase method after 6- to 7-day incubation with anti-protein gene product (PGP) 9.5 antibody as the primary antibody.
3. Developed networks of the myenteric plexus were observed in the fibromuscular layer near the outer surface. Distribution of the ganglia was clearly observed. Nerve strands of the patients were not significantly different from those of controls, but the number of ganglia of the patients decreased to one tenth of that of controls.
4. This distribution of nerve strands and ganglia in the bite duct wall choledochal cyst is similar to that in the intestinal wall of hypoganglionosis, which implies the mechanism of dilatation of the bile duct in patients with choledochal cyst is the same as that of megacolon in patients with hypoganglionosis.
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