| Project/Area Number |
10671176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
FUJISHIMA Masaki SHIGA UNIVERSITY OF MEDICAL SCIENCE, SURGERY, ASSOCIATED PROFESSOR, 医学部, 助教授 (50115771)
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Takashi SHIGA UNIVERSITY OF MEDICAL SCIENCE, SURGERY, ASSISTANT PROFESSOR, 医学部, 助手 (50263041)
FUJIYAMA Mineko SHIGA UNIVERSITY OF MEDICAL SCIENCE, ANATOMY, ASSOCIATED PROFESSOR, 医学部, 助教授 (10199359)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1999: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Enteric nervous system (ENS) / Organotypic culture / Retrograde transport / 5-HT3 / 5-HT4 receptor antagonist / ex vivo vascularly perfused rat model / motility / enteric nervous system (ENS) / 消化管壁内神経叢 / 逆行性神経トレーサー(Dil) / 器官培養 |
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| Research Abstract |
The projections of myenteric neurons within the myenteric plexus of the guinea-pig small intestine were established using retrograde tracing in organotypic culture. Three days after applying the fluorescent dye Dil to a single ganglion in the myenteric plexus, about 250 nerve cell bodies were labeled. Of these, 44% were located oral to the application site, 24% were located anally and 32% were located within 1 mm of this site. Two major morphological types of neurons could distinguished. Dogiel type I neurons had lamellar dendrites and single axons, Dogiel type I and II neurons accounted for 90% and 10% of all filled cells, respectively labeled nerve cell bodies were present up to 10 mm aboral to the Dil application site ; all neurons more than 2 mm oral had Dogiel type II features. Neurons with 1 mm oral and aboral to the Dil application site were located up to whole length circumferentially and were mainly Dogiel type I cells.
This work revealed remarkable polarity within the myenteric plexus, with a significant prevalence of myenteric neurons projecting anally for longer distances than those projecting orally. These long pathways are probably involved in the coordination of intestinal motility.
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