| Co-Investigator(Kenkyū-buntansha) |
TOOYAMA Ikuo Shiga University of Medical Science, Inst. of Molecular Neurobiology, Professor, 分子神経科学研究センター, 教授 (20207533)
KIMURA Hiroshi Shiga University of Medical Science, Inst. of Molecular Neurobiology, Professor and Doctor, 分子神経科学研究センター, 教授 (40079736)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
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| Research Abstract |
[BACKGROUND]
(1) In recent year, sepsis has accepted as a systemic inflammatory response syndrome (SIRS) that manifested changes in the body temperature, heart rate, respiratory rate and leukocyte count. These manifestations show clearly that humoral and neuronal regulation is deeply involved in the host response in this situation. Therefore, insights into changes in the central nervous system (CNS) are of value to further our understanding of pathophysiology involved in septic states. (2) C-Fos and c-Jun are inducible transcription factors (ITFs) encoded by c-fos and c-jun, respectively. The transcription activity of c-Jun are widely used as metabolic markers of activated neurons.
[PURPOSE]
A study was conducted determine how SIRS states affect CNS, using an antibody against c-Fos, c-Jun and phosphorylated c-Jun.
[RESULTS and DISCUSSION]
The immunohistochemical examination of rat brain at 8 h after SMAO demonstrated c-Fos, c-Jun and p-c-Jun IR in the specific nuclei including the central and basolateral amygdaloid nu. of the limbic system, the paraventricular hypothalamic nu., locas coeruleus, and nucleus tractus solitarious. The results could interpreted to mean that the limbic, hypothalamus, and brainstem system is activated in response to extensive gut necrosis and participate in neuroendocrine, autonomic, and emotional change. Moreover, it is suggested that attenuated c-Jun IR in the hippocampal dentate gyrus at 8 h after SMAO may be neuroanatomical evidence of memory disturbance in patients with severe abdominal sepsis.
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