| Project/Area Number |
10671190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
OKAJIMA Masazumi Hiroshima University, Medical Hospital, Research Associate, 医学部・附属病院, 助手 (90274068)
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| Co-Investigator(Kenkyū-buntansha) |
ASAHARA Toshimasa Hiroshima University, Medical Hospital, Professor, 医学部, 教授 (70175850)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | gastrointestinal motility / autonomic nerve preserving surgery / strain gauge force transducer / barostat / function preserving surgery / nitrogen oxide / gastric adaptive relaxation / pylorus preserving pancreatoduodenectomy / Strain Gauge Force Transducer |
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| Research Abstract |
The study was designed to analyze canine gastroduodenal motility using strain gauge force transducers (SGTs) or barostat, and to clarify the control mechanisms of the motility, especially the pylorus and the fundus.
We established the method to evaluate the canine pyloric opening using the SGTs. The relaxation and opening of the pyloric ring was observed synchronously with intense construction of the antrum during the phase III of normal dogs. As the same manner, phase III-like motility, which was not a typical periodic motility, was observed in the - pylorus-preserving pancreatoduodenectomy (PPPD) dogs. The phase III-like motility was induced by LeuィイD113ィエD1-motilin in both normal and PPPD dogs. Therefore. LeuィイD113ィエD1-motilin must improved dysmotility of the pylorus after PPPD.
We also clarified the role of cholinergic and nitrergic inputs in mechanically and chemically induced gastric relaxation in conscious dogs. When mechanical stimulation, was applied by insufflating the intragastric bag at constant rate, the recordings of barostat showed the steep linear increase in intragastric pressure to about 6 mmHg. After that, the pressure had continued to increase gradually. NィイD1GィエD1-monomethyl-L-arginine acetate (L-NMMA) increased the pressure/volume gradient, while atropine reduced it significantly. An oral intake of lipid as chemical stimulation rapidly increased the proximal gastric volume (150.0±50.2ml). Although the lipid-induced increase in gastric volume subsided after atropine i.v. (36.0±14.7 ml ; p<0.005), it was unaffected by treatment with L-NMMA (167.6±53.0ml). The study shows that nitrergic input is not necessary for chemically induced gastric relaxation, but it is necessary for mechanically induced one. On the other hand, continuous cholimergic input is necessary for the both.
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