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Study on Reconstruction of the Hepatic Artery and Superior Mesenteric Artery Using a Cryopreserved Graft

Research Project

Project/Area Number 10671194
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionEHIME UNIVERSITY

Principal Investigator

SATO Motomichi  Ehime University, School of Medicine, Department of Surgery II, Associate Professor, 医学部・附属病院, 講師 (50162491)

Co-Investigator(Kenkyū-buntansha) KASHU Yasuaki  Ehime University, School of Medicine, Department of Surgery II, Lecturer, 医学部, 助手 (40294813)
WATANABE Yuji  Ehime University, School of Medicine, Department of Surgery II, Associate Professor, 医学部, 講師 (20210958)
KAWACHI Kanji  Ehime University, School of Medicine, Department of Surgery II, Professor, 医学部, 教授 (90116020)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsHepatic ischemia / arterial reconstruction / cryopreservation / neutrophil / reperfusion injury / aorta / peritonitis / 肝動脈 / 血管移植 / 感染
Research Abstract

Reconstruction of the hepatic artery and superior mesenteric artery is challenging in hepatobiliary surgery. The purpose of this study is to evaluate the feasibility of cryopreserved arterial grafting and to eliminate intraoperative hepatic ischemia during these procedures.
1. The aorta of Wister rats was cryopreserved with a program-freezer. The preserved aorta was transplanted to a recipient rat using a microsurgical technique under the condition of purulent peritonitis. Histologically, the grafted aorta showed no infection and a normal process after cryopreserved arterial grafting, including temporary endothelial cell loss and intimal hyperplasia.
2. Reconstruction of the hepatic artery and superior mesenteric artery of pigs was difficult because of thick lymphatic channels.
3. Rats receiving 90-min hepatic ischemia were divided into group C (control), group U treated with Urge-8, an antineutrophil antibody andgroup F treated with FK506, an immunosuppressor. Liver enzymes, pH, interleukin-8 (IL-8), and arterial ketone body ratio (AKBR) were measured 1 hour and 1 week after hepatic reperfusion. Survival rates were compared. Histologic damages of the liver were studied and the number of hepatic neutrophil infiltration was counted using ASD staining. One week-survival rate was tended to be lower in group C (12/20) than in groups U (16/20) and F (15/19). One hour after reperfusion of the liver, levels of GOT, GPT, LDH, and IL-8, hepatic neutrophil counts, and degree of hepatic damages were higher in group C than in groups U and F. Most of these changes subsided 1 week after grafting.
4. Although hepatic tissue PO2 levels showed the presence of hepatic ischemia, it did not correlate to the degree of hepatic ischemic injury.
In conclusion, this study indicated the safety of cryopreserved arterial grafting in the presence of peritnitis and efficacy of netrophil suppression therapy for hepatic ischemia

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Motomichi Sato: "Temporary aortic shunt technique for porcine liver transplantation"Transplantation Proceedings. 30. 3714-3715 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen YX: "Protective effect of antineutrophil antibody、 Urge-8, on liver ischemia-reperfusion injury"Transplantation Proceedings. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen YX: "Cryopreserved aortic grafting in the presence of peritonitis"Transplantation Proceedings. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Han SL: "The rapeutic efficacy of an antineutrophil monoclonal antibody, Urge-8, against acute necrotizing pancreatitis in rats"Surgery. 119. 585-591 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Motomichi Sato, et al: "Temporary aortic shunt technique for porcine liver transplantation"Transplantation Proceedings. 30. 3714-15 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen YX. Et al: "Protective effect of antineutrophil antibody, Urge-8, on liver ischemia-reperfusion injury"Transplantation Proceedings. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chen YX. Et al: "Cryopreserved_aortic grafting in the presence_of peritonitis"Transplantation Proceedings. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Han SL, et al: "Therapeutic efficacy of an antineutrophil monoclonal antibody, Urge-8, against acute necrotizing pancreatitis in rats"Surgery. 119. 585-591 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Motomichi Sato: "Temporary aortic shunt technique for porcine liver transplantation."Transplantation Proceedings. 30. 3714-3715 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chen YX: "Protecter effect of antineutrophil antibody,Urge-8,on liver ischemia-neperfuion infung."Transplantation Proceedings. 印刷中. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chen YX: "Cryopreserved aortic grafting in the presence of peritonitis."Transplantation Proceedings. 印刷中. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Han SL: "Therapeutic efficacy of an antineutrophil monoclonal antibody Urge-8,agaimst acute necrotizing pancreatitis."Surgery. 119. 585-591 (1996)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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