Cloning and analysis of PTEN2 gene
| Project/Area Number |
10671212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Iwate Medical University |
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Principal Investigator |
ISHIDA Kaoru Surgery I, Iwate Medical University, Assistant Professor, 医学部, 教授 (70146041)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | PTEN / Akt / Real-time PCR / Gastric cancer / Breast cancer / 食道癌 / 抑制遺伝子 / AKT / BAD |
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| Research Abstract |
The cDNA fragments of PTEN2 cloned by RACE was a pseudogene located on the chromosome 9q. We investigated mutations and expression of PTEN mRNA in primary carcinomas and cell lines of the esophagus, stomach and breast. No mutations were found in esophageal and breast carcinomas, but only one mutation was confirmed in gastric cancer cell line. Inactivation of PTEN gene by hypermethylation was observed in a gastric and a breast cancer cell lines. 0verexpression was observed in two cell lines. Microdissected materials obtained from primary tumors showed inactivation of PTEN gene and overexpression of AKT gene. These carcinomas were all scirrhous type of carcinomas. These results indicated that inactivation of PTEN gene may play a significant role in these type of tumor cells.
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Report
(3 results)
Research Products
(17 results)
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[Publications] Ikeda,K., Terashima,M., Kawamura,H., Takiyama,I., Koeda,K., Takagane,A., Sato,N., Ishida,K., Iwaya,T., Maesawa,C., Yoshinari,H., Saito,K: "Pharmacokinetics of cisplatin in combined cisplatin and 5-fluorouracil therapy: a comparative study of three different schedules of cisplatin administration,"Jpn J Clin Oncol.. 28. 168-175 (1998)
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[Publications] Iwaya,T., Maesawa,C., Nishizuka,S., Suzuki,Y., *Sakata,K., Sato,N., lkeda,K., Koeda,K., Ogasawara,S., Otsuka,K., Kimura,Y., Aoki,K., Ishida,K., Saito,K., *Tamura G: "Infrequent frameshift mutations of polynucleotide repeats in multiple primary cancers affecting the esophagus and other organs."Genes Chromosomes Cancer. 23. 317-322 (1998)
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[Publications] Maesawa,C., *Tamura,G., Iwaya,T., Ogasawara,S., Ishida,K., Sato,N., Nishizuka,S., Suzuki,Y., Ikeda,K., Aoki,K., Saito,K., Satodate,R: "Mutations in the human homologue of the drosophila patched gene in esophageal squamous cell carcinoma."Genes Chromosomes Cancer. 21. 276-279 (1998)
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[Publications] Ishida,K., Koeda,K., Sato,N., Ikeda,K., Ohtsuka,K., Aoki,K., Kimura., Y., Iwaya,T., Uesugi,N., Nakamura,R: "Problems in neoadjuvant chemoradiotherapy preceding surgery for advanced squamous cell carcinoma of the thoracic esophagus."JJTCVS. 47. 262-266 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Maesawa,C., Ogasawara,S., lwaya,T., Ishida,K., Tamura,G,: "Advances in genetic research of esophageal carcinoma."J. Iwate Med. ASS.. 50(Suppl). 577-591 (1999)
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