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Cloning and analysis of PTEN2 gene

Research Project

Project/Area Number 10671212
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionIwate Medical University

Principal Investigator

ISHIDA Kaoru  Surgery I, Iwate Medical University, Assistant Professor, 医学部, 教授 (70146041)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPTEN / Akt / Real-time PCR / Gastric cancer / Breast cancer / 食道癌 / 抑制遺伝子 / AKT / BAD
Research Abstract

The cDNA fragments of PTEN2 cloned by RACE was a pseudogene located on the chromosome 9q. We investigated mutations and expression of PTEN mRNA in primary carcinomas and cell lines of the esophagus, stomach and breast. No mutations were found in esophageal and breast carcinomas, but only one mutation was confirmed in gastric cancer cell line. Inactivation of PTEN gene by hypermethylation was observed in a gastric and a breast cancer cell lines. 0verexpression was observed in two cell lines. Microdissected materials obtained from primary tumors showed inactivation of PTEN gene and overexpression of AKT gene. These carcinomas were all scirrhous type of carcinomas. These results indicated that inactivation of PTEN gene may play a significant role in these type of tumor cells.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Ikeda K, Ishida K, et al: "Pharmacokinetics of cisplatin in combined cisplatin and 5-5worol therapy: A comparative study of phree different schedulos of a splatin adrinisratin"Jpn J Clin Oncol.. 28. 168-175 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Imaya T., Ishida K, et al: "Infrequent frameshift mutations of polynucleotide repeats in multiple primary cancers affecting the esophagus and other organs"Genes Chromosomes Cancer. 23. 317-322 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maesawa, C, Ishida K et al: "Mutations in the human homologue of the drosphila patched gene in esophageal squamous cell carcinoma"Genes Chromosomes Cancer. 21. 276-279 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishida, K., et al: "Problems in neoadjurant chemoradio therapy proceding surgery for advanced squamous cell carcinoma of the thoracic esphagus"JJTCVS. 47. 262-266 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maesawa, C., Ishida K.,et al: "Advances in genetic research of esophageal cariciroma"J. Iwate Med. Ass.. 50. 577-591 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 中村隆二、石田薫 他: "進行性食道癌に対する化学放射線療法後の効果測定"臨床放射線. 44. 1515-1522 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ikeda,K., Terashima,M., Kawamura,H., Takiyama,I., Koeda,K., Takagane,A., Sato,N., Ishida,K., Iwaya,T., Maesawa,C., Yoshinari,H., Saito,K: "Pharmacokinetics of cisplatin in combined cisplatin and 5-fluorouracil therapy: a comparative study of three different schedules of cisplatin administration,"Jpn J Clin Oncol.. 28. 168-175 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwaya,T., Maesawa,C., Nishizuka,S., Suzuki,Y., *Sakata,K., Sato,N., lkeda,K., Koeda,K., Ogasawara,S., Otsuka,K., Kimura,Y., Aoki,K., Ishida,K., Saito,K., *Tamura G: "Infrequent frameshift mutations of polynucleotide repeats in multiple primary cancers affecting the esophagus and other organs."Genes Chromosomes Cancer. 23. 317-322 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maesawa,C., *Tamura,G., Iwaya,T., Ogasawara,S., Ishida,K., Sato,N., Nishizuka,S., Suzuki,Y., Ikeda,K., Aoki,K., Saito,K., Satodate,R: "Mutations in the human homologue of the drosophila patched gene in esophageal squamous cell carcinoma."Genes Chromosomes Cancer. 21. 276-279 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishida,K., Koeda,K., Sato,N., Ikeda,K., Ohtsuka,K., Aoki,K., Kimura., Y., Iwaya,T., Uesugi,N., Nakamura,R: "Problems in neoadjuvant chemoradiotherapy preceding surgery for advanced squamous cell carcinoma of the thoracic esophagus."JJTCVS. 47. 262-266 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maesawa,C., Ogasawara,S., lwaya,T., Ishida,K., Tamura,G,: "Advances in genetic research of esophageal carcinoma."J. Iwate Med. ASS.. 50(Suppl). 577-591 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishida,K: "Problems in necodjuvant chemoradiotherapy preceding surgery for advanced squamous cell carcinoma of the thovacic esophagus"Jpn.J.Thorac.Cardiovasc.surg.. 47・6. 262-266 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 前沢 千早 石田薫: "食道癌の家族集積性とその分子生物学的解析"G.I.Research. 8・1. 3-8 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikeda K,Ishida K.,et al.: "Pharmoco Kinetics of Cisplatin combined cisplatin and 5-Fluovouracil therapy:A comparative Study of thres different schedules of Cisplatin Adininistration" Jpn J Clin Oncol. 28(3). 168-175 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishida K.,et al.: "A few problenms of neoadjuvant chemoradiotherapy priorto surgery for advanced Squamous cell larinoma of the thoracic esophageal cancer" Jpn.J.Thorac.Cardiovasc.Surg.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mresauir C.,Ishida K.,et al.: "Mutations in the human homologue of the Droscphila parched gene in esophageal squamous cell cartinona" Genes Chrimosomes cancer. 21. 276-279 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwaya T.,Ishida T,et al.: "Infrequent frameshift mutations of polymucleoride nepeats in multiple Drimauy cancers affecting the esophagus and other organs" Genes Chromosomes cancer. 23. 317-322 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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