| Project/Area Number |
10671231
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kurume University |
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Principal Investigator |
OGATA Yutaka Kurume University, Medicine, Assistant professor, 医学部, 講師 (20177124)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUHARA Koji Kurume University, Medicine, fellow, 医学部, 助手 (70268902)
NAKAGAWA Motonori Kurume University, Medicine, fellow, 医学部, 助手 (00268947)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | ETS-1 / Angiogenesis / differential display / bFGF / VEGF / HOMEC / u-PA / MMPs / Angic genesis / Colorectal cancer / vaccular endothelial growth factor (VEGF) / Angiogenesis / 大腸癌 / 微小血管密度 / 血管内皮細胞 / differential display method / vascular endothelialgrowth factor(VEGF) |
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| Research Abstract |
Immunohistochemical studies using surgically resected specimens from colorectal cancer patients and esophageal cancer patients indicated that the VEGF expression by the tumor cells and its receptor KDR expression by the endothelial cells correlated with the microvessel density (MVD), and that the VEGF expression or its combined expression with KDR might be an independent prognostic factor. We also found a correlation between the ETS- 1 expression by the endothelial cells, and the VEGF and pyrirnidine nucleotide (PyNPase) expression in colorectal cancer. Moreover, the ETS- 1 expression was found to be an independent prognostic factor in colorectal cancer.
The expression of transcription factor ETS-1 was induced in endothelial cells (HOMEC) by bFGF. Differential display and western blot analysis showed that ETS-1 induced u-PA and MMP-l that related with angiogenesis. ETS-1 was found to induce gp96 mRNA in HOMEC.
These results suggested that ETS-l might be candidate as a target molecule for anti-angiogenic therapy. We have to evaluate a significance of heat shock protein gp96 in relation to angiogenesis.
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