| Project/Area Number |
10671248
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SHIRAISHI Shoichiro Shiga University of Medical Science, Second Surgery, Assistant Professor, 医学部, 助手 (30283568)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | deep hypothermia / circulatory arrest / delayed neuronal death / 遅発性神経細胞死 |
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| Research Abstract |
In animal models of transient ischemia, selective vulnerability and delayed neuronal death (DND) in the hippocampus have been extensively described. It is well known that the developing brains have tolerance to damage. In the present study, we investigated the critical stage of the damage of DND in the developing gerbils and the differences of glial reactions within a week after transient ischemia in various postnatal days were all perfused at 10 weeks-old (adult). Histopathological evaluation of DND was performed by cresil violet staining. DND was not seen before 3 weeks-old. Glial reactions were examined immunohistochemically by the antibodies against GFAP, Mac-1 and CNPase, for astrocyte, microglia and oligodendrocyte, respectively. The most interesting reactive changes were observed in GFAP immunostaining. Computer image analysis showed that area fraction of GFAP-positive structures in CAI region was significantly increased in both ischemic cases with DND in 3 days after ischemia, the numerous astrocytes were observed even in the lateral half of st. pyramidalis, in which DND was just occurring, as estimated in cresil violet staining. The findings suggest that the astrocytes play an important role in neuronal survival and death after ischemia in both cases with and without DND.
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