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Energy metabolism and reperfusion injury on cold ischemia of lungs.

Research Project

Project/Area Number 10671250
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

FUKUSE Tatsuo  Kyoto Univ. Faculty of Medicine, Lecturer, 医学研究科, 助手 (90263152)

Co-Investigator(Kenkyū-buntansha) HIRATA Toshiki  Kyoto Univ. Faculty of Medicine, Lecturer, 医学研究科, 助手 (70281110)
WADA Hiromi  Kyoto Univ. Faculty of Medicine, Professor, 医学研究科, 教授 (90167205)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
KeywordsLung preservation / Mitochondria / Energy metabolism / Aerobic metabolism / Anaerobic metabolism / ATP / Reperfusion injury / Trehalose / 肺再灌流障害
Research Abstract

We compared the preservative effects of a monosaccharide (glucose), disaccharides (trehalose, maltose, sucrose), and a trisaccharide (raffinose) to investigate whether or not the effects of saccharides on lung preservation depend on their molecular weight, energy-level maintenance and cytoprotective effects. The effects of saccharides may depend on their cytoprotective effect rather than on impairment activity or energy-level maintenance of the preserved lung. Trehalose proved to be superior to the other saccharides.
Energy depletion closely correlates with ischemia-reperfusion (I-R) injury in solid organs, but there has been no conclusion about the lungs that contain air. We investigated the alveolar state during cold storage and its relation to energy metabolism and I-R injury in an ex-vivo rat lung model. The lung was deflated (DEF group) or inflated with either room air (RA group) or nitrogen (N2 group) for six hours at 4℃, and reperfused or bronchoalveolar lavage fluid (BALF) was collected (N=6, each). Furthermore, the static lung compliance, the intrapulmonary levels of high energy phosphates, lactate and pyruvate were measured. The pulmonary functions of the DEF and N2 groups were significantly worse than those of the RA group. In the N2 group, the intrapulmonary levels of energy charge and pyruvate/lactate ratio were significantly lower than those in the DEF and RA groups, while there were no significant differences between the DEF and RA groups. In the DEF group, total protein and lactate dehydrogenase in the BALF were significantly higher while the static lung compliance was significantly lower compared with the N2 and RA groups. We concluded that aerobic metabolism would be essential for attenuating I-R injury of the lung, and inflation of the alveoli would be necessary for avoiding mechanical damage during re-expansion.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Fukuse T.: "Energy metabolism and mitochondrial damage during pulmonary preservation"Transplant Proc. 31. 1937-1938 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T.: "Role of saccharides on lung preservation"Transplantation. 68. 110-117 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T.: "Energy metabolism and reperfusion injury on warm and cold ischemia of inflated and deflated lungs"Transplant Proc. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T.: "Influence of deflated and anaerobic conditions during cold storage on rat lungs"American Journal of Respiratory and Critical Care Medicine. 160. 621-627 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T.: "Influence of alveolar gas during pulmonary preservation on reperfusion injury"Transplant Proc. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirata T.: "Reperfusion injury following cold preservation correlates with decreased levels of intrapulmonary high energy phosphates"Transplantation. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T: "Energy metabolism and mitochondrial damage during pulmonary preservation."Transplant Proc. 31. 1937-1938 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T: "Influence of deflated and anaerobic conditions during cold storage on rat lungs."American Journal of Respiratory and Critical Care Medicine. 160. 621-627 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka F, Fukuse T: "Prognostic significance of apoptotic index in completely resected non-small cell lung cancer."J Clin Oncol. 17. 2728-2736 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T: "Energy metabolism and reperfusion injury on warm and cold ischemia of inflated and deflated lungs."Transplant Proc. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T: "Influence of alveolar gas during pulmonary preservation on reperfusion injury."Transplant Proc. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirata T, Fukuse T: "Reperfusion injury following cold preservation correlates with decreased levels of intrapulmonary high energy phosphates."Transplantation. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukuse T: "Energy metabolism and mitochondrial damage during pulmonary preservation"Transplant Proc. 31. 1937-1938 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fukuse T: "Influence of deflated and anaerobic conditions during cold storage on rat lungs"American Journal of Respiratory and Critical Care Medicine. 160. 621-627 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka F: "Prognostic significance of apoptotic index in completely resected non-small cell lung cancer"J Clin Oncol. 17. 2728-2736 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fukuse T: "Energy metabolism and reperfusion injury on warm and cold ischemia of inflated and deflated lungs"Transplant Proc. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Fukuse T: "Influence of alveolar gas during pulmonary preservation on reperfusion injury"Transplant Proc. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirata T: "Reperfusion injury following cold preservation correlates with decreased levels of intrapulmonary high energy phosphates"Transplant Proc. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ueda M,Hirata T,Fukuse T,et al.: "Dibutylyl cyclic adenosine monophosphate atenuates damage in the ultrastructure of endothelial cells in 15-hour cold preserved rat lungs." Transplant Proc. 30. 53-55 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukuse T,Hirata T,et al.: "Energy metabolism and mitochondrial damage during pulmonary preservation." Transplant Proc. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirata T,Fukuse T,Wada H: "High-energy phosphates,mitochondria,and reperfusion injury in isolated rat lungs." Transplant Proc. 30. 3377-9 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukuse T,Hirata T,Wada H: "Role of saccharides on lung preservation" Transplantation. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ueda M,Hirata T,Fukuse T,et al.: "Mitochondrial Injuries in Rat Lungs Preserved for 17 Hours ; an Ultrastructural Study." European Surgical Research. (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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