| Project/Area Number |
10671255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | YAMAGATA UNIVERSITY |
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Principal Investigator |
TAKAHASHI Toshiki 2nd Dep. Of Surg. Medical School, Yamagata University, Associate Professor, 医学部・第二外科, 講師 (50263257)
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| Co-Investigator(Kenkyū-buntansha) |
OIZUMI Hiroyuki 2nd Dep. Of Surg. Medical School, Yamagata University, Associate Professor, 医学部・第二外科, 講師 (40250930)
MINOWA Takashi 2nd Dep. Of Surg. Medical School, Yamagata University, Assistant Professor, 医学部・第二外科, 助手 (50292420)
INUI Kiyoshige 2nd Dep. Of Surg. Medical School, Yamagata University, Assistant Professor, 医学部・第二外科, 助手 (70250941)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | transmyocardial laser revascularization / angiogenic factor / hepatocyte growth factor / ischemic heart disease / chronic ischemic myocardium / regional myocardial blood flow / cardiac function / レーサー心筋内血管新生術 / 慣性心筋虚血 |
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| Research Abstract |
Transmyocardial laser revascularization (TMLR) and intramural injection of angiogenic factor has been proposed to alleviate angina status for patients with ungraftable ischemic heart disease by means of possible mechanism such as angiogenesis. In this experimental study, we evaluated the efficacy of TMLR and intramural injection of human recombinant hepatocyte growth factor (HGF) (IIHGF). [Methods] Beagles were subjected to proximl LAD ligation. After one month of LAD ligation, TMLR, IIHGF and combination of TMLR and IIHGF were performed in each beagle group (TMLR group, IIHGF group, TMLR+IIHGF group). In TMLR+IIHGF group, TMLR was first performed and second HGF were injected into the myocardium around the TMLR channels. One month after each treatment, regional myocardial blood flow (MBF), regional cardiac function (SS) with or without dobutamine stress, and histochemical analysis were evaluated using colored microsphere for MBF, sonomicrometer for SS, and PCNA staining for neovascularization. [Results] TMLR and IIHGF improved MBF and SS in the LAD lesion. MBF and SS in the IIHGF group was significantly higher than those in TMLR group. Histologically, in the LAD lesion, the number of PCNA-positive arterioles was markedly increased in the IIHGF group as compared with those of the TMLR group. IIHGF in conjunction with TMLR improved the effect of TMLR on the MBF, the SS and angiogenesis in chronic ischemic myocardium. [Conclusion] Our data demonstrated that intramural injection of HGF showed superior improvement of the angigenesis and the intrinsic contractile state as compared with TMLR in chronic ischemic myocardium and enhanced the effect of TMLR markedly.
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