| Project/Area Number |
10671295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | SHIGA UNIVERSITY OF MEDICAL SCIENCE |
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Principal Investigator |
SUZUKI Fumio SHIGA UNIVERSITY OF MEDICAL SCIENCE, DEPARTMENT OF NEUROSURGERY, INSTRUCTOR, 医学部, 講師 (80171247)
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| Co-Investigator(Kenkyū-buntansha) |
MAKIURA Yaeko SHIGA UNIVERSITY OF MEDICAL SCIENCE, DEPARTMENT OF ANESTHEOLOGY, ASSISTANT, 医学部, 助手 (90293836)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | NEUROPLASTICITY / HIPPOCAMPAL GRANULE CELL / DIFFERENTIAL DISPLAY / NEURANAL HYPERTROPHY / NOVEL GENE |
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| Research Abstract |
Intrahippocampal injection of a subtoxic dose of kainate (KA) in mice has been shown to induce a dispersion and a hypertrophic change of granule cells of the dentate gyrus. These morphological changes are long-lasting with conspicuous increases of brain-derived neurotrophic factor (BDNF).
Whereas, the previous results indicated that BDNF was related only with the initiation of hypertrophy. Therefore, some other factors should exist to support this hypertrophic change. To find the other factors, in this study we investigated the messenger RNA expression in subacute and chronic phases after KA injection by differential display PCR.
Five bands were differentially displayed both in 2 and 8 weeks after KA injection and sequenced but four out of five were thought as non-specific. One remaining gene was 500 bp, and was homologous with KIAA0279 that was identified from human brain cDNA library. In situ hybridization studies revealed that the gene was expressed stronger in the hypertrophic granule cells. A Northern blot analysis showed that the gene existed not only in brain and but also in the other organs, and its length was about 2kbp. In the brain, the gene was expressed not only in the hypertrophic granule cells but also expressed moderately in the dentate granule cells, cerebeller granule cells, and weakly in the whole brain. Although the hypertrophy could not be induced in rat, the gene was expressed in rat like as mouse. Development study showed that the gene was observed in the cortical plate that reduced with the advancement of the development. These results suggested that this gene is not specifically related to the hypertrophy itself but also related generally to the neuronal development and plasticity.
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