| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
Observations were made as below after comparisons among mice homozygously (HO) or heterozygously (HE) deficient in platelet activating factor (PAF) receptor and wild type (WT) littermate mice.
1. Physiological parameters: Under halothane anesthesia, nonfasted male and female mice of the three groups (HO, HE, WT) showed no significantly different parameters with regards to body temperature, temporal muscle temperature, arterial blood gases, blood glucose, mean arterial pressure.
2. Infarct Volumes: Male and female mice of the above three groups underwent focal cerebral ischemia by inserting 11-12mm of 6-0 nylon filament from left common carotid artery according to Koizumi method. Twenty-four hours after onset of ischemia, the mice were sacrificed. Five 2 mm brain slices were stained with 2% 2, 3, 5-triphenyltetrazolium chloride and tissue injury volume was calculated by numerically integrating the injury areas in each slice. The ischemic injury size (mean+SEM mm3 )were 56±19 for WT, 55±16 for HO and 34±8 for HE. There were no statistically significant differences among three groups.
3. Cerebral Blood Flow (CBF): Focal CBF as measured by laser-Doppler flowmeter during insertion of nylon filament from left common carotid artery demonstrated no significant differences in reduction of CBF as compared to the baseline between HO and WT.
Conclusions: These data demonstrate negative evidence for the hypothesis that PAF is involved in brain injury after focal cerebral ischemia.
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