| Project/Area Number |
10671362
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
IHARA Koichiro Yamaguchi University Hospital, Lecturer, 医学部・附属病院, 講師 (90184788)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Soutetsu Yamaguchi University Hospital, Clinical Fellow, 医学部・附属病院, 医員(臨床)
KATSUBE Kousuke Yamaguchi University Hospital, Clinical Fellow, 医学部・附属病院, 医員(臨床)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | MUSCLE TRANSPLANTATION / SKELETAL MUSCLE / GROWTH FACTOR / BASIC FIBROBLAST GROWTH FACTOR |
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| Research Abstract |
We investigated the role of basic fibroblast growth factor (bFGF) in the regenerative process after free-muscle transfer. Using Wister rats, medial head of gastrocunemius muscle was prepared for the model of free-muscle. Neurorraphy of the sciatic nerve, which had been severed during the preparation, was performed. In the meantime, blood supply to the muscle was temporarily ceased for an approximately one hour. After accomplishing these preparation, the muscle was orthotopically placed into its original position.
The operated gastrocunemius muscles as well as those of the contraleteral side were harvested 4, 6, 8 weeks after surgery, and then preserved at - 80 degree of Celsius. Total RNA was excreted before an expression of bFGF mRNA was examined, using RT-PCR. While the expression of bFGF mRNA was observed at the 4th week after surgery, it subsided thereafter. The time was roughly equal to the reinnervation stage of the free-muscle. In the current study, any expression of bFGF mRNA was not demonstrated in the contralateral muscles.
The results implied that bFGF plays an important role in the recovery of the free-muscle at an early stage after the transplantation. Further study should be undertaken to investigate the expression of bFGF mRNA in detail, especially those within a few weeks after surgery.
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