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The interaction of IGF and IGF-binding proteins in repairing of cartilage tissue

Research Project

Project/Area Number 10671366
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNagasaki University

Principal Investigator

ENOMOTO Hiroshi  Nagasaki University School of Medicine, Assistant, 医学部, 助手 (90284679)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Shunichi  Nagasaki University School of Medicine, Professor, 医学部, 教授 (30200679)
MATSUMOTO Tomoko  Nagasaki University School of Medicine, Associate Professor, 医学部, 助教授 (30239107)
TOMONAGA Tadashi  Nagasaki University School of Medicine, Lecturer, 医学部・附属病院, 講師 (00264242)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordschondrocytes / Cartilage tissue / Insulin like growth factor / IGF binding protein / 再生
Research Abstract

Insulin-like growth factor-I (IGF-I) is an important anabolic factor for cartilage tissue and its action is in part regulated by IGF-binding proteins (IGFBPs). The object of this study was to investigate the effects of IGFBPs on IGF-I action and on binding of IGF-I to cells using a reproducible immortalized human chondrocyte culture model. Treatment of the C-28/I2 cells with IGF-I or des (1-3 ) IGF-I in serum-free medium stimulated cell proliferation in a dose-dependent manner. However, the effect of des (1-3) IGF-I was more potent, thereby suggesting that endogenously produced IGFBPs inhibited IGF action. The stimulatory effect of IGF-I was inhibited significantly by addition of IGFBP-3. Binding of [125I] IGF-I to the cells was displaced by IGFBP-3. Our findings indicate that the balance between IGFBPs, IGF receptor binding and its action on chondrocyte proliferation may modulate cartilage tissue repair.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Matsumoto T: "the inhibitory effects of IGF binding protein-3 on IGF action in cultured human chondrocytes"Biomed Eng Appl Basis Comm. 11. 1183-1186 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yonekura A: "Transforming growth factor-b stimulates articular chondrocytes cell growth through p44/42 MAP kinasa (ERK) activation"Endocrine J. 46. 545-553 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chamoun D: "Transcriptional and posttranscriptional regulation of intraovarian insulin-like growth factor-binding proteins by interleukin-1 beta (IL-1 beta): evidence for IL-1 beta as antiatretic principal"Endocrinology. 140. 3488-3495 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Osaki M: "Regulation of c-fos Gene induction and Mitogenic effect of transforming growth factor-b1 in rat articular chondrocyte"Endocrine J. 46. 253-261 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T: "Decrea ed lev 1s of IGF binding protein-3 in serum from children with Perthes' disease"Acta Orthopedica Scandinavica. 69. 125-128 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T: "Increased levels of IGF-I and IGFBP-3 in synovial fluids of patients with rheumatoid arthritis"Endocrine Journal. 45. 141-144 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T: "The inhibitory effects of IGF binding protein-3 on IGF action in cultured human chondrocytes"Biomed Eng Appl Basis Comm. 11. 11183-11186 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yonekura A: "Transforming growth factor-b stimulates articular chondrocytes cell growth through p44/42 MAP kinasa (ERK) activation"Endocrine J. 46. 545-553 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chamoun D: "Transcriptional and posttranscriptional regulation of intraovarian insulin-like growth factor- binding proteins by interleukin-1 bet a (IL-1 beta): evidence for IL- 1beta as antiatretic principal"Endocrinology. 140. 3488-3495 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Osaki M: "Regulation of c-fos Gene induction and Mitogenic effect of transforming growth factor-b1 in rat articular chondrocyte"Endocrine J. 46. 253-261 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T: "Decreased levels of IGF binding protein-3 in serum from children with Perthes' disease."Acta Orthop Scand. 69. 125-128 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T: "Increased levels of IGF-I and IGFBP-3 in synovial fluids of patients with rheumatoid arthritis"Endocrine Journal. 5(suppl). 141-144 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T: "The inhibitory effects of IGF binding protein-3 on IGF action in cultured human chondrocytes"Biomed Eng Appl Basis Comm. 11. 11183-11186 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chamoun D: "Transcriptional and posttranscriptional regulation of intaovarian insulin-iike factor binding proteins by interleukin-1 beta (IL-1 beta): evidence for IL-1 beta as antiatretic principal"Enddocrinology. 140. 3488-3495 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yonekura A,: "Transforming growth factor-β stimualates articular chondrocytes cell growth through P44/42 MAP kinasa (ERK) activation"Endocrine J. 46. 545-553 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Okano K: "Effect of 22-Oxa-1,25 Dihydroxy Vitamin D_3 on Human Thyroid Cancer Cell growth"Endocrine J. 46. 243-252 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osaki M: "Regulation of c-fos Gene Induction and Mitogenic Effect of Transforming Crowth Factor-β1 in Rat Articular chondrocyte"Endocrine J. 46. 253-261 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto T,: "Increased levels of IGF-I and IGFBP-3 in synoyial fluids of patients with rheumatoid arthritis." Endocrine Journal. 45. S141-S144 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto T,: "Decreased levels of IGF binding protein-3 in serum from children with Perthes' disease." Acta Orthopedica Scandinavica. 69. 125-128 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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