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The Origin of the repaired cells and function of mesenchymal stem cell

Research Project

Project/Area Number 10671375
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research Institutionbasic research

Principal Investigator

TAKAI Shinro  assistant prof., 医学部, 講師 (10226730)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsmesenchymal stem cell / differentiation / transgenic animal / in situ hybridization / bone marrow / Ligament / fibroblast / healing / 靱帯 / 自家骨軟骨移植 / トランスジェニックラット / 粘弾性特性
Research Abstract

In this study the origin of the repaired cells and the function of mesenchymal stem cells in tissue healing were examined. The transplantation model to eliminate the immunological rejection of the donor cells by the host was developed in order to follow transplanted cells in vivo. First, various kinds of cells were transplanted into tissues to prove the integrity of this model in following the transplanted cells. Then, the differentiation of mesenchymal stem cells was studied using this transplantation model. In this study, a female transgenic rat was used as a donor, and a female wild-type rat was used as a recipient. Since the DNA between these rats is theoretically coincident except for transgenes, transplanted cells could be followed in the recipient tissues by in situ hybridization against transgenes of transgenic rats cells. Furthermore, due to the use of the transgenic rat, which originally contains transgenes in the whole body, the introduction of markers is not necessary. Usin … More g this novel transplantation model with immunohistochemistry against BrdU, it was found that transplanted synovial cells survive and proliferate in the joint for at least 4 weeks (Trans ORS 1998, Cell Tissue RES 1999). By combining the newly developed micro-indentator, it was also found that donor cells in the transplanted osteochondral fragment caused the enchondral ontogenesis and helped in the recovery of viscoelastic properties (Trans ORS 1999). This transplantation model therefore was beneficial in performing multidisciplinary study on the repair of the tissue, using biological and biomechanical approaches. On the other hand, bone marrow is well known as a source of mesenchymal stem cells. In this study, bone marrow cells of the transgenic rat were transplanted into the injured medial collateral ligament (MCL) of the knee in the wild-type rat. The differentiation of these cells was morphologically examined. As a result, bone marrow cells were demonstrated to differentiate into fibroblasts. These cells were also found to migrate into the midsubstance and assisted in constitute the healing MCL (Hilton head workshop 1999). Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Nobuyoshi Watanabe et al: "Amethod of tracking donor cells after simufated aistologous transpontation study using synovial cell of transgenic rats"Cell & Tissue Research. 298. 519-525 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nobuyoshi Watanabe et al.: "A method of tracking donor cells after simulated autologous transplantation : a study using synovial cells of transgenic rats"Cell Tissue Res. 298. 519-525 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nobuyoshi Watanabe et al: "A method of tracking donor cell after simulated actologius transplantation : a study using synovial cell of transgenicrats"Cell & Tissue Research. 298. 519-525 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] TAKENAKA.N TAKAI Setal: "BEHAVIOR OF INTRINSIC CELLS in situ AND EXTRAINSIC CELLS SUPPLIFD BY AUTOLOGOUS OSTEOCHONDRAL TRANSPLANTATION" Transaction of the 45th Aunual Meeting. 45. 333 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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