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Optical analyses of ischemic dysfunction in cortex-hippocampus neural networks using membrane potential images

Research Project

Project/Area Number 10671406
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNiigata University

Principal Investigator

FUJIWARA Naoshi  Niigata University School of Medicine Assistant professor, 医学部, 助教授 (70181419)

Co-Investigator(Kenkyū-buntansha) TANAKA Eiichiro  Kurume University School of Medicine Lecturer, 医学部, 講師 (80188284)
WATANABE Ippei  Niigata University Medical Hospital Assistant, 医学部・附属病院, 助手 (00251819)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsbrain slices / potential sensitive dye / membrane potential / propagation of neuronal excitation / transient brain ischemia / ischemic neuronal damages / 大脳皮質 / 海馬 / 膜電位感受性色素 / 神経細胞興奮 / 脳虚血 / スプレディングデプレション
Research Abstract

1) The propagation of neuronal excitation was optically recorded in gerbil hippocampal and cortical slices which were dyed with a potential sensitive fluorescence dye, RH 414. When the Schaffer collaterals of hippocampal CA1 region were electrically stimulated, the propagation of membrane depolarization extended to both stratum radiatum and stratum oriens. In cortical slices, neuronal excitation evoked by a stimulation onto the layer IV axially propagated to the layer II, and then extended over the same layer.
2) The extent of neuronal depolarization was also observed in gerbil brain slices which were prepared 18-20 hr after 4 min of transient forebrain ischemia. When the Schaffer collaterals were stimulated, the extent of neuronal depolarization spread only within the stratum radiatum and the propagation of neuronal excitation to the stratum pyramidale and stratum oriens was inhibited. Thus, neuronal dysfunction might already occur 18-20 hr after the transient ischemia, although degeneration of pyramidal neurons was not found.
3) The cortical spreading depression which was evoked by high-KィイD1+ィエD1 stimulation did not significantly affect to the extent of neuronal depolarization evoked by electrical stimulation in cortical slices.
4) Gerbils pre-treated with or without 2 min of forebrain ischemia (pre-conditioning) were insulted to 5 min of forebrain ischemia. The propagation of neuronal excitation was improved in hippocampal slices from pre-conditioned gerbils in comparison with those from gerbils without pre-conditioning. Hence, the pre-conditioning may induce the tolerance against functional ischemic neuronal damages.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 藤原直士 他3名: "ニューロン膜脱分極によるアシドーシスの発生機序とその意義"蘇生. 17・1. 23-30 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan R-Z 他3名: "Intracellular acidification induced by membrane depolarization in rat hippocampal slices ; roles of intracellular Ca_<2+> and glycolysis"Brain Research. 780. 86-94 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 藤原直士 他3名: "膜電位画像による脳虚血後の神経興奮伝搬の解析"Brain Hypoxia. 12. 25-29 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan R-Z 他6名: "Thiopental inhibits increases in [Ca^<2+>] induced by membrane depolarization, NMDA receptor activation and ischemia in ray hippocampal and cortical slices"Anesthesiology. 89. 456-466 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan R-Z 他5名: "Differential inhibitory effects of thiopental, thiamylal and phenobarbital on both voltage-gated calcium channels and NMDA receptors in rat hippocampal slices"Br J Anesth. 81. 932-939 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 藤原直士: "光学的方法による細胞内pH測定"新潟大学医療短期大学部紀要. 6. 297-302 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan RZ,他4名: "大脳皮質視覚野の虚血性神経伝達障害に対するNMDAレセプター遮断薬の効果"Brain Hypoxia. 13. 25-31 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujiwara N, Okamoto Y, Zhan R-Z, Shimoji K: "Optical images of excitation spreading in hippocampal slices following cerebral ischemia (in Japanese)"Brain Hypoxia. 12. 25-29 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujiwara N, Zhan R-Z, Taga K, Shimoji K: "Acidosis induced by membrane depolarization of neurons : its mechanism and physiological significance (in Japanese)"Sosei. 17(1). 23-30 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan R-Z, Fujiwara N, Tanaka E, Shimoji K: "Intracellular acidification induced by membrane depolarization in rat hippocampal slices : roles of intracellular CaィイD12+ィエD1 and glycolysis"Brain Res. 780. 86-94 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan R-Z, Fujiwara N, Yamakura T, Taga K, Shimoji K: "Differential inhibitory effects of thiopental, thiamylal and phenobarbital on both voltage-gated calcium channels and NMDA receptors in rat hippocampal slices"Br J Anaesth. 81. 932-939 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan R-Z, Fujiwara N, Endoh H, Yamakura T Taga K, Fukuda S, Shimoji K: "Thiopental inhibits increase in [CaィイD12+ィエD1]ィイD2iィエD2 induced by membrane depolarization, NMDA receptor activation, and ischemia in rat hippocampal and cortical slices"Anesthesiology. 89. 456-466 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujiwara N: "Measurements of intracellular pH by optical method"Bull Coll Biomed Technol Niigata Univ. 6(3). 297-302 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan R-Z, Fujiwara N, Taga K, Fukuda S, Shimoji K: "The different protective effects of NMDA receptor antagonism on in vitro ischemia-induced neuro-transmission failure in the visual cortex of the rat"Brain Hypoxia. 13. 25-31 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhan RZ, 他4名: "大脳皮質視覚野の虚血性神経伝達障害に対するNMDAレセプター遮断薬の効果"Brain Hypoxia. 13. 25-31 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 藤原直士 他3名: "ニューロン膜脱分極によるアシドーシスの発生機序とその意義" 蘇生. 17・1. 23-30 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Zhan R-Z 他3名: "Intracellular acidification induced by membrane depolarization in rat hippocampal slices: roles of intracellular Ca^<2+> and glycolysis" Brain Research. 780. 86-94 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤原直士 他3名: "膜電位画像による脳虚血後の神経興奮伝搬の解析" Brain Hypoxia. 12. 25-29 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Zhan R-Z 他6名: "Thiopental inhibits increases in[Ca^<2+>]induced by membrane depolarization,NMDA receptor activation and ischemia in ray hippocampal and cortical slices" Anesthesiology. 89. 456-466 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Zhan R-Z 他5名: "Differential inhibitory effects of thiopental,thiamylal and phenobabital on both voltage-gated calcium channels and NMDA receptors in rat hippocampal slices" Br J Anesth. 81. 932-939 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤原直士: "光学的方法による細胞内pH測定" 新潟大学医療技術短期大学部紀要. 6. 297-302 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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