• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Histochemical Study on Onset and Recovery Mechanism of Hepatic Injury Induced by Inhalation Anesthesia in Rats.

Research Project

Project/Area Number 10671415
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionOkayama University

Principal Investigator

YAMADA Teruo  Medical School, Okayama University Assistant, 医学部, 助手 (00033225)

Co-Investigator(Kenkyū-buntansha) NOMURA Takako  Medical School, Okayama University Assistant, 医学部, 助手 (20116437)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordshistology / immunohistology / in situ hybridization / inhalation anesthetic / hepatic injury / apoptosis / 肝細胞 / 組織化学 / アポトーシス / ネクローシス / アクチン / rRNA / 分子生物学
Research Abstract

The liver was histochemically examined to study each effect of enflurane (E) , halothane (H) , isoflurane (I), and sevoflurane (S) in phenobarbital-treated (PB-group) and non-treated (N-group) rats. Under 100, 21 and 10% oxygen, rats were exposed to four anesthetics at 1 MAC, respectively, for 2h and the liver was removed at fixed intervals until 7 days after the exposure. Embedded in paraffin and sliced, the liver was stained by various ways of staining to study time-course changes in lobular cell structure. Marked lobular cell change was found only in PB-group under 10% oxygen, while under any other condition no or a little but soon recoverable change was found after the exposure. In PB-group, the liver tissues exposed to each anesthetic were negatively stained with PAS in 1/3 to 2/3 area from the central vein to the portal vein and rRNA disappeared soon after the exposure. The cells in the area negatively stained with PAS degenerated into vacuoles from 12h to 2 days after the exposure. By 7 days after the exposure, the vacuoles disappeared recovering to the normal lobular structure. No anesthetics induced cell proliferation until 7 days and apoptosis dispersed around the vacuoles 1 to 2 days after the exposure. Infiltrated cells appearing in the degenerated area were of macrophage. The degree of cell degeneration induced by each anesthetic showed the following order : H> E > I> S.As to the cause of hepatic injury after inhalation anesthesia, various theories have been reported, but from our results, the main cause is considered that each anesthetic extremely decreases hepatic blood flow volume resulting in the state of ischemia in the central vein area. As to the recovery mechanism, it is considered that after degenerated cells are removed to the central vein by hepatic blood flow, the cells in the portal vein area are rearranged along the hepatic cell cords.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] T.Yamada, T.Nomura, Y.Miki, S.Kanada and J.Sasaki: "Histochemical and Immunohistochemical Studies on Hepatocyte Injury by Halothane Exposure and Its Recovery Mechanism in Rat"Jornal of Anesthesia. 15(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Yamada, T.Nomura, Y.Miki, S.Kanada and J.Sasaki: "Histochemical Studies on Hepatic injury by Sevoflurane, Isoflurane and Enflurane, and Its Recovery Mechanism in Rats"Jornal of Anesthesia. 15(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Teruo Yamada, Takako Nomura, Yukari Miki, Shigeto Kanda and Junzo Sasaki: "Histochemical and Immunohistochemical Studies on Hepatocyte Injury by Halothane Exposure and Its Recovery Mechanism in Rat"Jornal of Anesthesia. Vol.15 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Teruo Yamada, Takako Nomura, Yukari Miki, Shigeto Kanda and Junzo Sasaki: "Histochemical Studies on Hepatic Injury by Sevoflurane, Isoflurane and Enflurane, and Its Recovery Mechanism in Rats"Jornal of Anesthesia. Vol.15 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi