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The effects of propofol, a novel intravenous anesthetic agent, an regulation of vascular tone in isclateo rabbit pulmonary arteries

Research Project

Project/Area Number 10671437
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNagoya City University

Principal Investigator

KATSUYA Hirotada  Nagoya city university medicine professor, 医学部, 教授 (20040561)

Co-Investigator(Kenkyū-buntansha) ITOH Takeo  Nagoya city university medicine professor, 医学部, 教授 (70159888)
NAKANO motomi  Nagoya city university medicine assistant, 医学部, 助手 (30275143)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Keywordspropofol / prostacyclin / HィイD22ィエD2OィイD22ィエD2 / rabbit mesenteric arteries / hyperpolarize / rabbit pulmonary arteries / H_2O_2(過酸化水素) / 等尺性張力 / 肺動脈 / in vitro / 血管周囲神経刺激
Research Abstract

By measuring isometric contractions, intracellular concentration of CaィイD12+ィエD1([CaィイD12+ィエD1]ィイD2iィエD2) and membrane potential changes, we found that propofol had many actions on agonist-induced CaィイD12+ィエD1-mobilization mechanisms in smooth muscle cells of rabbit mesenteric arteries : (1) inhibition of CaィイD12+ィエD1 influxes through L-type CaィイD12+ィエD1 channels, (2) inhibition of agonist-induced CaィイD12+ィエD1 release from intracellular CaィイD12+ィエD1 storage sites, (3)inhibition of [CaィイD12+ィエD1]ィイD2iィエD2 removal mechanisms. In addition, propofol inhibits the synthesis of prostacyclin, but not of EDHF, in endothelial cells of these arteries. Propofol is known to be a scavenger against reactive oxygen species (ROS). However, the physiological function of these ROS has not been clarified in resistance vessels. Therefore, the effect of HィイD22ィエD2OィイD22ィエD2, one of major ROS in vascular tissues, was first examined on regulation of vascular tones in rabbit mesenteric arteries. As a result, we found that (1) HィイD22ィエD2OィイD22ィエD2 attenuated the contraction induced by norepinephrine in endothelium-intact and -denuded preparations, (2) HィイD22ィエD2OィイD22ィエD2 increases the synthesis of prostanoids (PGEィイD22ィエD2, PGIィイD22ィエD2) in both preparations, (3) HィイD22ィエD2OィイD22ィエD2 hyperpolarizes a smooth muscle cell membrane through an activation of glibenclamide-sensitive KィイD2ATPィエD2 channels. Now, we are trying to examine the action of propofol on these HィイD22ィエD2OィイD22ィエD2-induced responses in rabbit mesenteric arteries. In a future, using rabbit intrapulmonary arteries, we are planning to clarify the physiological and pathophysiological roles of HィイD22ィエD2OィイD22ィエD2 and also to examine the action of propofol on the HィイD22ィエD2OィイD22ィエD2-induced responses.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Nami Imura: "Effect of propofol on norepinephrine-induced increases in [Ca^<2+>] and farce smooth muscle of the rabbit mesenteric resistance artery"Anesthesiology. 88. 1566-1578 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akiko Yamashita: "Inhibitory effect of Propsofol on Acetylcholine-induced, Endothelium-depenclent Relaxation and Prostocyclin Synthesis in Rabbit Mesenteric Resistance Arteries"Anesthesiology. 91. 1080-1089 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 伊藤猛雄: "硝酸薬のすべて(Part I 硝酸薬の作用機序)"硝酸薬のすべて. 35-59 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nami Imura: "Effect of propofol on norepinephrine-induced increases in [CaィイD12+ィエD1]ィイD2iィエD2 and force in smooth muscle of the rabbit mesenteric resistance artery"Anesthesiology. 88. 1566-1578 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akiko Yamashita: "Inhibitory Effects of Propofol on Acetylcholine-induced, Endothelium-dependent Relaxation and Prostacyclin Synthesis in Rabbit Mesenteric Resistance Arteries"Anesthesiology. 91. 1080-1089 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nami Imura: "Effect of propofol on noreplnephrine-induced increases in [Ca^<2+>] : and force in smoch muscle of the rabbit mesenteric resistance artery"Anesthesiology. 88. 1566-1578 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akiko Yamashita: "Inhibitory Effects of Propofol on Acetylcholine-induced, Endothelium-dependent Relaxation and Prostacydin Synthesis in Rabbit Mesenteric Resistance Arteries"Anesthesiology. 91. 1080-1089 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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