| Project/Area Number |
10671454
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Occupational Environmental Health (UOEH) |
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Principal Investigator |
KAMOCHI Masayuki University of UOEH, The faculty of medicine, assistant professor, 医学部, 講師 (90204643)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGEMATSU Akio Uinversity of UOEH, The faculty of medicine, professor, 医学部, 教授 (30037428)
WATANABE Hiroyuki Uinversity of UOEH, The faculty of medicine, instructor, 医学部, 助手 (90220920)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | lung injury / endotoxin / sepsis / C12MDP / vascular permeability / liposome / chlodronate / エンドトキシン |
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| Research Abstract |
The liposome of Cl2MDP was made using rotary evaporator. The C12MDP was a kind gift from Dr. Esser (Boeringer-Manheim).
At first we examined the effect of the depletion of the alveolar macrophages on LPS-induced lung microvascular permeability in the rat. The alveolar macrophages were depleted by the intratracheal injection of the liposome of C12MDP. The control animals were injected PBS-liposome intratrachealy.
We found that the LPS-induced lung microvascular permeability of the rat with depleted alveolar macrophages was significantly lower than that of the control animal at 24h after i.p. LPS injection. When we injected the liposome of C12MDP intravenously, the number of the alveolar macrophages of the rat did not change, on the other hand the intravascular macrophage (monocyte) were completely depleted.
The LPS-induced lung vascular permeability of the rat with depleted intravascular macrophages was significantly higher than the control animal.
In these experiments, we concluded that the alveolar macrophages play an important role in sepsis induced lung injury. However, we could not know the reason why the lung vascular permeability increased in the rat with depleted intravascular macrophages by intravenous injection of the liposome of C12MDP. The mechanism of the increase of the lung vascular permeability in the rat injected C12MDP intravenously is now under investigation.
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