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Morphological study about glutamate neural transmission in the micturitional center in central nervous system.

Research Project

Project/Area Number 10671455
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionHokkaido University

Principal Investigator

AMEDA Kaname (1999-2000)  Hokkaido Univ., Grad.School of Med., Inst., 大学院・医学研究科, 助手 (60271657)

柴田 隆 (1998)  北海道大学, 医学部附属病院, 助手 (50292032)

Co-Investigator(Kenkyū-buntansha) KAKIZAKI Hidehiro  Hokkaido Univ., Medical, Hospital, Lec., 医学部・附属病院, 講師 (10241324)
飴田 要  北海道大学, 医学部, 助手 (60271657)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsglutamate transporter / NMDA receptor / spinal cord injury / lower urinary tract obstruction / micturition / development / in situ hybridization / グルタミン酸トランスポータ / グルタミン酸 / トランスポーター / 脊髄 / in situ ハイブリダイゼーション / グルタミン酸受容体 / NMDA型受容体 / AMPA型受容体
Research Abstract

Glutamate is a major excitatory neurotransmitter in the mammalian central nervous system. Physiological studies have shown that lower urinary tract function is regulated through the glutamate receptors at the level of spinal and supraspinal cord. To know the ontogenic change of NMDA receptor subunit mRNAs and function in developing spinal micturitional system, we employed double labeling technique of retrograde neuronal tracing and in situ hybridization for detecting NMDA subunit mRNAs in developing preganglionic neurons (PGNs) in the rat, which innervate bladder muscle. We also examined expression patterns of NMDA subunit mRNAs and glutamate transporter subtype mRNAs in lower urinary tract obstruction model and spinal cord injury model, which are both important human pathologies in micturition system.
The PGNs showed strong signals for NR1 subunit mRNA at every stage during development to adult. Moderate signals for the NR2B and NR2D subunit mRNAs were found in PGNs at postnatal day 7. However, their expression levels decreased gradually during development, with minimal expressions in Adult. These results show that there are dynamic changes in the expression of the NR2 subunit mRNAs in the PGNs during development. PGNs seem to change their property through the NMDA receptor during development. This temporal expression of functional NMDA receptors during early development might contribute to the establishment of the adult-type micturitional neural circuit through activity-dependent synaptic plasticity and refinement. There were no significant changes in the expression of glutamate receptor and transporter molecules both in lower urinary tract obstruction and spinal cord injury models.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report

Research Products

(1 results)

All Other

All Publications (1 results)

  • [Publications] T.Shibata: "Different Expressions of α-amino-3-hydroxy-5-methy1-4-isoxazole Propianic Acid and N-methyl-D-aspaqtate Receptor Subunit in RNAs Between Visceromotor and Somatomotor Neurons of the Rat Lunbosacrd Spinal cord" The Journal of Comparatice Neurology. 404. 172-182 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-03-31   Modified: 2016-04-21  

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