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Study of epithelial-stromal interaction in estrogen-induced proliferation

Research Project

Project/Area Number 10671458
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionGunma University

Principal Investigator

YUASA Hisako  Gunma university, school of medicine Assistant, 医学部, 助手 (50240148)

Co-Investigator(Kenkyū-buntansha) YAMANAKA Hidetoshi  Gunma university, school of medicine Professor, 医学部, 教授 (70110393)
FUKABORI Yoshitatsu  Gunma university, school of medicine Lecturer, 医学部, 講師 (90199167)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsestrogen / estrogen receptor / seminal vesicle / TGF-β1 / epithelium / stroma / interaction / androgen / 間質細胞 / 基底細胞 / 平滑筋 / TGF-β / サイトケラチン / シラスチック・チューブ
Research Abstract

I. Investigation on immature rat : (1) In seminal vesicle (SV) of estrogen-treated castrated rats, proliferative epithelial cells, had multilayer features, were cytokeratin (34βE12 : basal cell specific antibody) positive and TGF-β1 positive (Tohoku J Exp Med, 1999) (2) We were raised antibody of estrogen receptor (ER)β and observed ERβ localization in male and female reproductive organs using immunohistochemical means. ERβ was strong staining in ventral prostate (VP), however it was weak staining in SV. In our previous study, were demonstrated that ERα was immunopositive in SV and was negative in VP. These findings suggested that ERα and ERβ might covered for functions of each other, SV and VP (Kitakanto Med J, 1999). (3) The expression of ERα, ERβ mRNA were qualified by real-time PCR. ERα mRNA was induced by castration or castration plus estrogen-treatment at a level 3.9 and 7.6-fold higher than untreatment in SV, respectively, but not in VP. In contrast, ERβ mRNA was induced at a le … More vel 1.9 and 10.3-fold by castration or castration plus estrogen-treatment, respectively in VP, but not in SV (J Urol, 1999). (4) The expression of ERα, ERβ mRNA were demonstrated by rapid In-situ hybridization. In SV, ERα mRNA was observed to peak at the 2nd day after successive estrogen administration and then decreased at the 7th day On the other hand, in SV ERβ mRNA observed to peak at the 3rd day and completely suppressed at the 7th day.
II. Investigation on adult rats : In VP, ERβ mRNA level decreased at 12hrs after castration and to 3.7% of normal level at 48hrs. On the other hand, in VP, ERβ mRNA increased at 6hrs after injection of androgen and up to 4 to 5-fold at 12hrs. These datas were suggested that ERβ had androgen dependency (J Urol, 2000).
III. Investigation on cultured epithelial cells of human prostate : Level of ARmRNA on epitlelial cells increased about 7-fold with exposure to estrogen at a concentration of 10-8M, compared with cells without estrogen. On the other hand, ERβ mRNA increased about 7-fold with exposure to androgen at a concentration of 10-7M, compared with cells without androgen. These results suggested that AR-ER cross-talk existed in the human prostate (J Urol, 1999). Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] H.Yuasa: "Localization of estrogen receptor-β in rat tissue by immunohistochemistry"Kitakanto Med. J.. 49(6). 409-412 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Yuasa: "Immunohistrochemical characteristics of estrogen receptorα positive cells in glandular epithelium of the rat seminal vesicle"Tohoku J. Exp. Med.. 187(1). 25-35 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Yuasa: "Usefulness of the estrogen releasing silastic tubing-Estrogenic effect on seminal vesicle in immature castrated rats-"Kitakanto Med. J.. 48(1). 15-18 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 深堀 能立: "-アンドロロジーの最近の進歩-前立腺ホルモン支配の分子機構と細胞増殖因子"臨床泌尿器科. 53(10). 772-778 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuasa H.: "Localization of estrogen receptor-β in rat tissue by immunohistochemistry."Kitakanto Med J. 49(6). 409-412 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuasa H.: "Immunohistochemical characteristics of estrogen receptorα positive cells in glandular epithelium of the rat seminal vesicle."Tohoku J Exp Med. 187(1). 25-35 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuasa H.: "Usefulness of the estrogen releasing silastic tubing -Estrogenic effect on seminal vesicle in immature castrated rats-."Kitakanto Med J. 48(1). 15-18 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fukabori Y.: "-Recent progress in andrology- Molecule mechanism and cell growth factors in prostate hormone control"J Clin Urol. 53(10). 772-778 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Yuasa: "Localization of estrogen receptor-β in rat tissue by immunohistochemistry"Kitakanto Med.J.. 49(6). 409-412 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Yuasa: "Immunohistochemical characteristics of estrogen receptorα positive cells in glandular epithelium of the rat seminal vesicle"Tohoku J. Exp. Med.. 187(1). 25-35 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Yuasa: "Usefulness of the estrogen releasing silastic tubing-Estrogenic effect on seminal vesicle in immature castrated rats-"Kitakanto Med.J.. 48(1). 15-18 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 深堀能立: "-アンドロロジ-の最近の進歩-前立腺ホルモン支配の分子機構と細胞増殖因子"臨床泌尿器科. 53(10). 772-778 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuasa H: "Immunohistochemical characteristics of estrogen receptor α positive cells in glandular epithelium of the rat seminal vesicle." Tohoku J Exp Med. 187(1)(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yuasa H.: "Usefulness of the estrogen releasingsilastic tubing -Estrogenic effect on seminal vesicle in immature castrated" Kitakanto Med J. 48(1). 15-18 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yuasa H.: "Effects of sex hormones on the tissue localization of nuclear estrogen receptor positively stained cells in the seminal vesicle of immature castrated rats." Tohoku J Exp Med. 181(2). 297-309 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukabori Y.: "Stimulative effect of transforming growth factor-β on collagen synthesis by human prostatic stromal cells in vitro" Int J Urol. 4. 597-602 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山中英寿: "新しいエストロゲンレセプター、ERβの発見" Prostate Cancer-New poimts of view-3(山中英寿監修). 10 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山中英寿: "前立腺・精嚢細胞の増殖制御-上皮・間質,growth factors-" CURRENT THERAPY. 14(11). 2013-2016 (1996)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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