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Distribution and aging changes of αィイD21ィエD2-adrenoceptor subtype mRNA in human renal artery.

Research Project

Project/Area Number 10671459
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionUNIVERSITY of TOKYO

Principal Investigator

MORIYAMA Nobuo (1999)  The Univ. Tokyo, Assist. Prof., 医学部・附属病院, 講師 (80143501)

黒岡 雄二 (1998)  東京大学, 医学部附属病院, 助手 (80161777)

Co-Investigator(Kenkyū-buntansha) 森山 信男  東京大学, 医学部・附属病院, 講師 (80143501)
亀山 周二  東京大学, 医学部・附属病院, 講師 (90186015)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsHuman renal artery / αィイD21ィエD2-adrenoceptor / αィイD21ィエD2-adrenoceptor mRNA / in situ hybridization / RNAase protection assay / ヒト腎実質
Research Abstract

This study was intended to quantify the amounts of the αィイD21ィエD2-adrenoceptor subtype mRNAs in human renal artery, and demonstrate the distribution of receptor subtype responsible for the contraction of renal artery. RNase protection assay showed that the mean amount of αィイD21aィエD2-mRNA was much more than that of αィイD21bィエD2- or αィイD21dィエD2-mRNAs in both the main and branch renal arteries. However, the abundance of αィイD21aィエD2-mRNA in human renal artery was much less than our previous data in the prostate. By in situ hybridization, all αィイD21ィエD2 subtype mRNAs were localized in the smooth muscle cells of the tunica media of the artery, and the distribution pattern of these three mRNAs in the main artery was the same as in the branch artery. However, the intensity of signals for αィイD21dィエD2 and αィイD21bィエD2 antisense RNAs probes were lower than that for αィイD21aィエD2 antisense RNA probe. In the functional study, concentration-response curves to noradrenaline pretreated with KMD-3213, an αィイD21A/LィエD2-adrenoceptor selective antagonist, seemed to be biphasic curves. Chloroethyclonidine (CEC) failed to inactivate the noradrenaline-induced contraction and prazosin showed relatively low affinity with a pAィイD22ィエD2 value of 8.8. These data suggest that the αィイD21A/LィエD2-adrenoceptor primarily mediates those responses to noradrenaline in this artery. The other αィイD21ィエD2-adrenoceptor subtypes could also mediate the secondary contractile response to noradrenaline in this artery.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Suzuki, Y, Moriyama, et al.: "Age-related change of the role of α_<1L>-adrenoceptor in canine urethral smooth muscle"Gen. Pharmacol.. 33. 347-354 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takahashi, M, Taniguchi, T, Murata, S, Okada K, Moriyama N, et al.: "New α_1-adrenoceptor antagonist, JTH-601, shows more than 10 times higher affinity for the human prostates than arteries"J. Urol.. 161. 1350-1354 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishimatsu, H, Hamada, K, Moriyama, N, et al.: "Contractile responses to α_<1L>-adrenoceptor agonists in isolated human male and female urethra"Br. J. Urol.. 84. 515-520 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Taki, N, Taniguchi, T, Okada, K, Moriyama, N, et al.: "Evidence for predominant mediation of α_<1L>-adrenoceptor in the tonus of whole urethra of women"J. Urol.. 162. 1829-1832 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki, Y, Moriyama, N, et al.: "The role of α_<1L>-adrenoceptor in rat urinary bladder; Comparison between young adult and aged rats"Life Sciences. 65. 2553-2559 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Moriyama, N, et al.: "Distribution of α_<1L>-adrenoceptor subtype mRNA and identification of subtype responsible for renovascular contraction in human renal artery"Life Sciences. 66. 915-926 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki, Y., Moriyama, et al.: "Age-related change of the role of αィイD21LィエD2-adrenoceptor in canine urethral smooth muscle."General Pharmacologyr. 33. 347-354 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takahashi, M., Taniguchi, T., Murata, S., Okada, K., Moriyama, N., et al.: "New αィイD21ィエD2-adrenoceptor antagonist, JTH-601, shows more than 10 times higher affinity for the human prostates than arteries."Journal of Urology. 161. 1350-1354 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishimatsu, H., Hamada, K., Moriyama, N., et al.: "Contractile responses to αィイD21LィエD2-adrenoceptor agonists in isolated human male and female urethra."British Journal of Urology. 84. 515-520 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Taki, N., Taniguchi, T., Okada, K., Moriyama, N., et al.: "Evidence for predominant mediation of αィイD21LィエD2-adrenoceptor in the tonus of whole urethra of women."Journal of Urology. 162. 1829-1832 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki, Y., Moriyama, N., et al.: "he role of αィイD21LィエD2-adrenoceptor in rat urinary bladder ; Comparison between young adult and aged rats."Life Sciences. 65. 2553-2559 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Moriyama, N., et al.: "Distribution of αィイD21ィエD2-adrenoceptor subtype mRNA and identification of subtype responsible for renovascular contraction in human renal artery."Life Sciences. 66. 915-926 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,Y., Moriyama, et al.: "Age-related change of the role of α_<1L>-adrenoceptor in canine urethral smooth muscle"Gen. Pharmacol.. 33. 347-354 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takahashi, M., Taniguchi, T., Murata, S., Okada, K., Moriyama, N., et al.: "New α_1-adrenoceptor antagonist, JTH-601, shows more than 10 times higher affinity for the human prostates than arteries"J. Urol.. 161. 1350-1354 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishimatsu, H., Hamada, K., Moriyama, N., et al.: "Contractile responses to α_<1L>-adrenoceptor agonists in isolated human male and female urethra"Br. J. Urol.. 84. 515-520 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taki, N., Taniguchi, T., Okada, K., Moriyama, N., et al.: "Evidence for predominant mediation of α_<1L>-adrenoceptor in the tonus of whole urethra of women"J. Urol.. 162. 1829-1832 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki, Y., Moriyama, N., et al.: "The role of α_<1L>-adrenoceptor in rat urinary bladder ; Compparison between young adult and aged rats"Life Sciences. 65. 2553-2559 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Moriyama, N., et al.: "Distribution of α_1-adrenoceptor subtype mRNA and identification of subtype responsible for renovascular contraction in human renal artery"Life Sciences. 66. 915-926 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Konno,N.,Mori,M.,Kurooka,S.,et al.: "Carcinosarcoma in the region of the female urethra." Int.J.Urol.4. 229-231 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurooka,Y.,et al.: "Distribution of α_1-adrenoceptor subtype mRNAs in human renal cortex." Br.J.Urol.83. 299-304 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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